Miller A F, Brudvig G W
Department of Biochemistry, Brandeis University, Waltham, MA 02254.
Biochim Biophys Acta. 1991 Jan 3;1056(1):1-18. doi: 10.1016/s0005-2728(05)80067-2.
This guide is intended to aid in the detection and identification of paramagnetic species in Photosystem II membranes, by electron paramagnetic resonance spectroscopy. The spectral features and occurrence of each of the electron paramagnetic resonance signals from Photosystem II are discussed, in relation to the nature of the moiety giving rise to the signal and the role of that species in photosynthetic electron transport. Examples of most of the signals discussed are shown. The electron paramagnetic resonance signals produced by the cytochrome b6f and Photosystem I complexes, as well as the signals from other common contaminants, are also reviewed. Furthermore, references to seminal experiments on bacterial reaction centers are included. By reviewing both the spectroscopic and biochemical bases for the electron paramagnetic resonance signals of the cofactors that mediate photosynthetic electron transport, this paper provides an introduction to the use and interpretation of electron paramagnetic resonance spectroscopy in the study of Photosystem II.
本指南旨在通过电子顺磁共振光谱法，辅助检测和鉴定光系统II膜中的顺磁物种。讨论了来自光系统II的每个电子顺磁共振信号的光谱特征和出现情况，涉及产生该信号的部分的性质以及该物种在光合电子传递中的作用。展示了所讨论的大多数信号的示例。还综述了细胞色素b6f和光系统I复合物产生的电子顺磁共振信号，以及来自其他常见污染物的信号。此外，还包括了对细菌反应中心开创性实验的参考文献。通过回顾介导光合电子传递的辅因子的电子顺磁共振信号的光谱和生化基础，本文介绍了电子顺磁共振光谱法在光系统II研究中的应用和解读。
