Yang L, Zhang Y, Cui F Z
Biomaterials Laboratory, Department of Material Science & Engineering, Tsinghua University, 100084, People's Republic of China.
Biomed Mater. 2007 Mar;2(1):21-5. doi: 10.1088/1748-6041/2/1/004. Epub 2007 Jan 12.
Two types of mineral-related matrix vesicle, multivesicular body (MVB) and monovesicle, were detected in the skeletal bone of zebrafish. Transmission electron microscopy and energy dispersive spectroscopy (EDS) analyses of the vesicular inclusions reveal that both types of vesicles contain calcium and phosphorus, suggesting that these vesicles may be involved in mineral ion delivery for the bone mineralization of zebrafish. However, their size and substructure are quite different. Monovesicles, whose diameter ranges from 100 nm to 550 nm, are similar to the previously reported normal matrix vesicles, while MVBs have a larger size of 700-1000 nm in nominal diameter and possess a substructure that is composed of smaller vesicles with their average size around 100 nm. The presence of mineral-related MVBs, which is first identified in zebrafish bone, indicates that the mineralization-associated transportation process of mineral ions is more complicated than is ordinarily imagined.
在斑马鱼的骨骼中检测到两种与矿物质相关的基质小泡，即多泡体（MVB）和单泡。对小泡内含物的透射电子显微镜和能量色散光谱（EDS）分析表明，这两种小泡都含有钙和磷，这表明这些小泡可能参与了斑马鱼骨矿化的矿物质离子传递。然而，它们的大小和亚结构有很大不同。单泡的直径范围为100纳米至550纳米，与先前报道的正常基质小泡相似，而MVB的标称直径较大，为700 - 1000纳米，并且具有由平均大小约为100纳米的较小小泡组成的亚结构。首次在斑马鱼骨中鉴定出与矿物质相关的MVB，这表明矿物质离子的矿化相关运输过程比通常想象的更为复杂。
