Cao Chengbo, Zhu Fanglian, Yu Xueli, Wang Qin, Wang Chuandong, Li Baolu, Lv Ronghui, Li Musen
School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, People's Republic of China.
Biomed Mater. 2008 Mar;3(1):015002. doi: 10.1088/1748-6041/3/1/015002. Epub 2007 Dec 19.
Poly(lactic acid) (PLA) was modified by maleic anhydride (MAH), then the resultant MAH modified PLA (MPLA) was acylated with ethylenediamine (EDA), so EDA-MAH modified PLA (EMPLA) was prepared. The results of DSC, FT-IR and NMR testified that MAH and EAD were successfully introduced into the original polymer. The hydrophilicity of EMPLA was considerably increased compared with that of PLA. The degradation experiment showed that the introduction of EDA into the original polymer could neutralize the carboxyl end groups of the degradation products. The results of SEM and MTT of rat osteoblasts cultured in vitro showed that the cytocompatibility and cell adhesion of the modified materials were significantly increased compared with the original polymer, especially EMPLA; the number of cells were obviously increased and cells attached firmly to the material; these were ascribed to the EDA neutralizing the carboxyl end groups of the degradation products.
聚乳酸（PLA）用马来酸酐（MAH）进行改性，然后将所得的MAH改性PLA（MPLA）与乙二胺（EDA）进行酰化反应，从而制备出EDA-MAH改性PLA（EMPLA）。差示扫描量热法（DSC）、傅里叶变换红外光谱（FT-IR）和核磁共振（NMR）的结果证明MAH和EDA成功引入到原始聚合物中。与PLA相比，EMPLA的亲水性显著提高。降解实验表明，将EDA引入原始聚合物中可以中和降解产物的羧基端基。体外培养大鼠成骨细胞的扫描电子显微镜（SEM）和噻唑蓝比色法（MTT）结果表明，与原始聚合物相比，改性材料的细胞相容性和细胞黏附性显著提高，尤其是EMPLA；细胞数量明显增加且细胞牢固地附着在材料上；这些归因于EDA中和了降解产物的羧基端基。
