Xiang Yan, Wang YuanLiang, Luo Yanfeng, Zhang Bingbing, Xin Juan, Zheng Danfang
Research Center of Bioinspired Materials Science and Engineering, Bioengineering College, Chongqing University, Chongqing 400044, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2011 Aug;28(4):753-7.
The main objective of this study was to observe the adhesion, proliferation and differentiation of mouse osteblast-like MC3T3-E1 cells cultured on maleic anhydride-modified poly(D,L-lactic acid) (MPLA) and poly(D,L-lactic acid) (PDLLA) polymers, and to evaluate the cytocompatibility of MPLA polymer. The effects of MPLA and PDLLA polymers on the morphology, adhesion, proliferation, the content of total cellular protein, alkaline phosphatase (ALP) activity and the content of Ca of MC3T3-E1 cells were explored. These results indicated that MC3T3-E1 cells on MPLA polymer adhered and spread more fully. On MPLA polymer, the proliferation, total protein content, ALP activity, Ca content of the cells were significantly higher than those of the cells on PDLLA polymer (P < 0.01). It was concluded that MPLA polymer could promote the adhesion, spreading, proliferation and the synthesis of protein of osteoblasts, and also induced the differentiation and mineralization of osteoblasts, suggesting that MPLA polymer might have the better cytocompatibility than PDLLA.
本研究的主要目的是观察在马来酸酐改性聚（D，L-乳酸）（MPLA）和聚（D，L-乳酸）（PDLLA）聚合物上培养的小鼠成骨样MC3T3-E1细胞的黏附、增殖和分化情况，并评估MPLA聚合物的细胞相容性。探讨了MPLA和PDLLA聚合物对MC3T3-E1细胞形态、黏附、增殖、总细胞蛋白含量、碱性磷酸酶（ALP）活性和钙含量的影响。这些结果表明，MPLA聚合物上的MC3T3-E1细胞黏附更充分且铺展良好。在MPLA聚合物上，细胞的增殖、总蛋白含量、ALP活性、钙含量均显著高于PDLLA聚合物上的细胞（P<0.01）。结论是，MPLA聚合物可促进成骨细胞的黏附、铺展、增殖和蛋白质合成，还能诱导成骨细胞分化和矿化，表明MPLA聚合物可能比PDLLA具有更好的细胞相容性。
