Jelinsky Scott A, Lake Spencer P, Archambault Joanne M, Soslowsky Louis J
Wyeth Discovery Research, 87 Cambridge Park Drive, Cambridge, MA 02140, USA.
Clin Orthop Relat Res. 2008 Jul;466(7):1612-7. doi: 10.1007/s11999-008-0270-z. Epub 2008 May 6.
Rest is a common treatment for overuse injuries, but its effectiveness on gene expression has not been systematically evaluated under controlled experimental conditions. We asked whether genes regulated in the supraspinatus tendon as a result of overuse would return to normal levels after 2 or 4 weeks of rest. We used a rat model of tendon overuse that generates reproducible changes in the histology, geometry, gene expression, and mechanical properties consistent with an overuse injury. Animals were subjected to the overuse protocol for 2 or 4 weeks followed by either 2 or 4 weeks of rest. Microarray analysis was used to measure global changes in gene expression after the overuse plus rest protocol. Genes upregulated as a result of the overuse returned to near normal levels after rest in most animals. The biochemical composition of the tendon was similar to normal after the imposed rest period, except for slightly lower collagen content. These results suggest as little as 2 weeks of rest is often sufficient to recover from the molecular and biochemical effects of 2 and 4 weeks of overuse in this rat model.
休息是治疗过度使用损伤的常用方法，但在可控的实验条件下，其对基因表达的有效性尚未得到系统评估。我们研究了因过度使用而在冈上肌腱中被调节的基因在休息2周或4周后是否会恢复到正常水平。我们使用了一种肌腱过度使用的大鼠模型，该模型在组织学、几何学、基因表达和力学性能方面产生了与过度使用损伤一致的可重复变化。动物先进行2周或4周的过度使用方案，然后进行2周或4周的休息。微阵列分析用于测量过度使用加休息方案后基因表达的整体变化。在大多数动物中，因过度使用而上调的基因在休息后恢复到接近正常水平。在规定的休息期后，肌腱的生化组成与正常情况相似，只是胶原蛋白含量略低。这些结果表明，在这个大鼠模型中，仅2周的休息通常就足以从2周和4周过度使用所造成的分子和生化影响中恢复过来。
