吸烟者与非吸烟者口腔黏膜脱落细胞学标本中AgNOR蛋白的定量分析。
Quantitative analysis of AgNOR proteins in exfoliative cytology specimens of oral mucosa from smokers and nonsmokers.
作者信息
Campos Fontes Patrícia, Marques Corrêa Gustavo Henrique, Scholz Issa Jaqueline, Almeida Janete Dias
机构信息
Postgraduation Program in Oral Biopathology, São José dos Campos Dental School, UNESP-São Paulo State University, São José dos Campos, São Paulo, Brazil.
出版信息
Anal Quant Cytol Histol. 2008 Feb;30(1):16-24.
OBJECTIVE
To determine the cell proliferation rate and possible effects of cigarette smoking on the oral mucosa lining through analysis of silver-stained nucleolar organizer regions (AgNORs) in exfoliative cytology specimens.
STUDY DESIGN
Exfoliative cytology was performed on the left side of the border of the tongue and of the floor of the mouth in 25 smoking patients and 25 nonsmoking patients. The inclusion criterion for smokers was the consumption of more than 20 cigarettes per day for a minimum of 30 years.
RESULTS
The slides were stained by histochemical AgNOR method. In the nonsmoking group the mean number of AgNORs per nucleus was 2.732 +/- 0.236 in the tongue border and 2.918 +/- 0.195 in the floor of the mouth. In smoking patients the mean number of AgNORs per nucleus was 3.372 +/- 0.375 in the tongue border and 3.245 +/- 0.237 in the floor of the mouth.
CONCLUSION
The results suggest higher cell proliferation quantified by the histochemical AgNOR technique in exfoliative cytology specimens obtained from the oral mucosa lining of smokers presenting no clinical alterations.
目的
通过对脱落细胞学标本中银染核仁组成区（AgNORs）的分析，确定细胞增殖率以及吸烟对口腔黏膜的可能影响。
研究设计
对25名吸烟患者和25名非吸烟患者的舌缘和口底左侧进行脱落细胞学检查。吸烟者的纳入标准是每天吸烟超过20支，且至少持续30年。
结果
玻片采用组织化学AgNOR法染色。在非吸烟组中，舌缘每核AgNORs的平均数为2.732±0.236，口底为2.918±0.195。在吸烟患者中，舌缘每核AgNORs的平均数为3.372±0.375，口底为3.245±0.237。
结论
结果表明，在无临床改变的吸烟者口腔黏膜脱落细胞学标本中，通过组织化学AgNOR技术量化的细胞增殖较高。
Zotero 插件