Vyas Tarun, Verma Parul, Abidullah Mohammed, Kushwaha Sandhya Singh, Sahoo Pradyumna Kumar, Priyadarshini Smita R, Subudhi Santosh Kumar, Rana Vivek
Department of Oral Medicine and Radiology, RR Dental College, Udaipur, Rajasthan, India.
Department of Conservative Dentistry and Endodontics, IDS Sehora, Jammu and Kashmir, India.
Ann Afr Med. 2018 Oct-Dec;17(4):210-214. doi: 10.4103/aam.aam_69_17.
Bidi smoking is a serious health hazard which is common throughout South Asia and parts of the Middle East. It has been strongly implicated to various benign and malignant lesions of oral cavity and oropharynx. These tobacco-filled leaves deliver more than three times the amount of nicotine, carbon monoxide, and tar as cigarettes which exert injurious effects on cells reflected in terms of accelerated proliferative activity in normal oral mucosal cells.
This study aimed to compare the exfoliated cells from the oral mucosa of bidi smokers and nonsmokers, with emphasis on proliferative activity.
Exfoliative smears were obtained from the oral mucosa of forty participants (twenty nonsmokers and twenty smokers) with age group ranging from 30-80 years, in and around Barwala (Haryana). The cytologic smears were evaluated using Papanicolaou (PAP) stain and AgNOR in order to evaluate the presence of cytological alterations suggestive of inflammation, dysplasia, keratinization, and proliferative activity of epithelial cells. Only PAP Class I and Class II smears were observed.
Comparison of the mean number of AgNORs showed a significant difference between nonsmokers and smokers. Inflammatory alterations were found in 70% of smokers and 55% of nonsmokers. A significant difference in proliferative activity was observed between smokers and nonsmokers classified as PAP Class II.
A significant difference of AgNORs/nucleus was observed between bidi smokers and nonsmokers.
比迪烟吸食是一种严重的健康危害，在南亚和中东部分地区很常见。它与口腔和口咽的各种良性和恶性病变密切相关。这些填充烟草的叶子所释放的尼古丁、一氧化碳和焦油含量是香烟的三倍多，对细胞产生有害影响，表现为正常口腔黏膜细胞增殖活性加速。
本研究旨在比较比迪烟吸烟者和非吸烟者口腔黏膜的脱落细胞，重点关注增殖活性。
从巴瓦拉（哈里亚纳邦）及其周边年龄在30至80岁的40名参与者（20名非吸烟者和20名吸烟者）的口腔黏膜获取脱落涂片。使用巴氏（PAP）染色和核仁组成区嗜银蛋白（AgNOR）对比细胞学涂片进行评估，以评估提示炎症、发育异常、角化和上皮细胞增殖活性的细胞学改变的存在情况。仅观察巴氏I级和II级涂片。
AgNORs平均数量的比较显示非吸烟者和吸烟者之间存在显著差异。70%的吸烟者和55%的非吸烟者发现有炎症改变。在分类为巴氏II级的吸烟者和非吸烟者之间观察到增殖活性存在显著差异。
比迪烟吸烟者和非吸烟者之间观察到细胞核AgNORs存在显著差异。
