Chin Khin K, Trevithick-Sutton Colleen C, McCallum Jeremy, Jockusch Steffen, Turro Nicholas J, Scaiano J C, Foote Christopher S, Garcia-Garibay Miguel A
Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-1569, USA.
J Am Chem Soc. 2008 Jun 4;130(22):6912-3. doi: 10.1021/ja800926v. Epub 2008 May 7.
Singlet oxygen quantum yields generated by excited state aromatic amino acids (tryptophan, tyrosine, phenylalanine), N-acetylated amino acids (N-acetyl-tryptophan, N-acetyl-tyrosine, N-acetyl-phenylalanine), and from selected proteins and immunoglobulins have been quantified by time-resolved phosphorescence measurements. A small, but significant, quantum yield found for proteins and immunoglobulins demonstrates that molecular oxygen can diffuse through the polypeptide matrix and can be sensitized by residues buried within the folds of protein structure.
通过时间分辨磷光测量，已对由激发态芳香族氨基酸（色氨酸、酪氨酸、苯丙氨酸）、N-乙酰化氨基酸（N-乙酰色氨酸、N-乙酰酪氨酸、N-乙酰苯丙氨酸）以及选定的蛋白质和免疫球蛋白产生的单线态氧量子产率进行了量化。在蛋白质和免疫球蛋白中发现了一个虽小但显著的量子产率，这表明分子氧可以扩散穿过多肽基质，并可被埋藏在蛋白质结构折叠内部的残基敏化。
