仲醇氧化的单向生物氢转移
One-way biohydrogen transfer for oxidation of sec-alcohols.
作者信息
Lavandera Iván, Kern Alexander, Resch Verena, Ferreira-Silva Bianca, Glieder Anton, Fabian Walter M F, de Wildeman Stefaan, Kroutil Wolfgang
机构信息
Research Centre Applied Biocatalysis c/o Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Graz, Austria.
出版信息
Org Lett. 2008 Jun 5;10(11):2155-8. doi: 10.1021/ol800549f. Epub 2008 May 7.
Quasi-irreversible oxidation of sec-alcohols was achieved via biocatalytic hydrogen transfer reactions using alcohol dehydrogenases employing selected ketones as hydrogen acceptors, which can only be reduced but not oxidized. Thus, only 1 equiv of oxidant was required instead of a large excess. For the oxidation of both isomers of methylcarbinols a single nonstereoselective short-chain dehydrogenase/reductase from Sphingobium yanoikuyae was identified and overexpressed in E. coli.
通过使用醇脱氢酶并以选定的酮作为氢受体进行生物催化氢转移反应，实现了仲醇的准不可逆氧化，这些酮只能被还原而不能被氧化。因此，仅需1当量的氧化剂，而非大量过量的氧化剂。对于甲基甲醇的两种异构体的氧化，从鞘氨醇单胞菌中鉴定出一种单一的非立体选择性短链脱氢酶/还原酶，并在大肠杆菌中进行了过表达。
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