An R, Li H, Han Z, Shao Y, Liu S, Kong X
Division of Avian Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, PR China.
Acta Virol. 2008;52(1):23-30.
Five ORFs in the genome of Duck enteritis virus (DEV) corresponding to UL31, UL32, UL33, UL34, and UL35 genes of Herpes simplex virus 1 (HSV-1) were amplified by a modified "targeted gene walking" PCR, cloned, and sequenced. UL33, UL34, and UL35 genes were oriented from the left to the right of genome, while UL31 and UL32 had an opposite orientation. A comparison of deduced amino acid sequences of the DEV ORFs with their alphaherpesvirus homologs showed well-conserved regions except for the UL34 and UL35 genes. Phylogenetic analysis revealed that DEV was closer to the genus Mardivirus than to any other genus of the subfamily Alphaherpesvirinae. Based on this evidence, we proposed to assign DEV to the subfamily Alphaherpesvirinae.
通过改良的“靶向基因步移”PCR扩增、克隆并测序了鸭肠炎病毒（DEV）基因组中与单纯疱疹病毒1型（HSV-1）的UL31、UL32、UL33、UL34和UL35基因相对应的5个开放阅读框（ORF）。UL33、UL34和UL35基因在基因组中从左向右排列，而UL31和UL32的排列方向相反。DEV的ORF推导氨基酸序列与其α疱疹病毒同源物的比较显示，除了UL34和UL35基因外，存在保守区域。系统发育分析表明，DEV与马立克病毒属的亲缘关系比与α疱疹病毒亚科的任何其他属更近。基于这一证据，我们建议将DEV归入α疱疹病毒亚科。
