Liu Shengwang, Li Huixin, Li Yang, Han Zongxi, Shao Yuhao, An Rui, Kong Xiangang
Division of Avian Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, PR China.
Intervirology. 2008;51(3):151-65. doi: 10.1159/000146865. Epub 2008 Jul 22.
To understand the evolutionary relationship of duck enteritis virus (DEV) in the family Herpesviridae and the genome organization of DEV.
A modified 'targeted gene walking PCR' method was used to amplify the unknown gene of DEV Clone-03 strain adjacent to a short stretch of known sequence. The assembled unknown fragment was confirmed to be a homologue of herpesviruses by BLAST analysis and comparison with other herpesviruses homologues. Open reading frames (ORFs) were determined and analyzed by Gene Runner software and an online transcriptional element search engine. Two evolutionary analysis approaches were used to conduct phylogenetic trees of DEV in relation to herpesviruses based on the deduced amino acid sequences.
A genomic fragment encompassing 25,625 nt and containing 16 ORFs was amplified, determined, and compared with homologues in other herpesviruses. Phylogenetic trees indicated that DEV and other herpesviruses generated from one ancestor and DEV were grouped into the subfamily Alphaherpesvirinae. In most cases, DEV showed a close relationship with the genus Mardivirus, but formed a single branch.
Partial genomic organization and phylogenetic analysis in the present study provides evidence that DEV was a member of the subfamily Alphaherpesvirinae and should be assigned as a single group.
了解疱疹病毒科中鸭肠炎病毒（DEV）的进化关系及DEV的基因组结构。
采用改良的“靶向基因步移PCR”方法扩增DEV Clone - 03株与一段已知短序列相邻的未知基因。通过BLAST分析并与其他疱疹病毒同源物比较，确认组装的未知片段为疱疹病毒的同源物。使用Gene Runner软件和在线转录元件搜索引擎确定并分析开放阅读框（ORF）。基于推导的氨基酸序列，采用两种进化分析方法构建DEV与疱疹病毒的系统发育树。
扩增、确定了一个包含25625个核苷酸且含有16个ORF的基因组片段，并与其他疱疹病毒的同源物进行了比较。系统发育树表明，DEV与其他疱疹病毒起源于同一个祖先，且DEV被归入α疱疹病毒亚科。在大多数情况下，DEV与马立克病毒属关系密切，但形成一个单独的分支。
本研究中的部分基因组结构和系统发育分析提供了证据，证明DEV是α疱疹病毒亚科的成员，应归为一个单独的组。
