[小窝蛋白-1的表达与绒毛膜癌的侵袭]

Liu Hui-ning, Cai Jing-ting, Lin Qiu-hua, He Ke-ren, Yu Rong

Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University, Changsha, China.

Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2008 Apr;33(4):331-7.

OBJECTIVE

To investigate the association between the expression of caveolin-1(CAV-1) and the invasion of choriocarcinoma, and to explore the effect of CAV-1 small interfering RNA(siRNA) on the invasion of choriocarcinoma cell line JEG-3.

METHODS

(1) Matrigel invasion assay and 3-(4,4)-dimethylthiahiazo (-z-yl)-3,5-di-phenytetrazoliumormide (MTT) assay were used to examine the difference in invasion and proliferation ability between JEG-3 cells and JAR cells;(2) Expression of caveolin-1 gene in the human chorionic villi tissues and chorionicnoma cell lines (JEG-3 cells and JAR cells) were detected by semi-quantitative RT-PCR. (3) The effect of CAV-1 siRNA transfection on the expression of CAV-1 mRNA, and the invasion and proliferation ability of JEG-3 cells were measured by RT-PCR, Matrigel invasion assay and MTT assay.

RESULTS

(1) The invasion ability of JEG-3 cell line was stronger than that of JAR cell line (P<0.05), but the difference in proliferation ability between JAR and JEG-3 was not obvious (P>0.05);(2) The expression of caveolin-1 gene in chorionicnoma cell lines was significantly stronger than that in the human normal chorion(P<0.05), and the expression of caveolin-1 gene in JEG-3 cells was stronger than that in the JAR cells (P<0.05). The data suggested that there was significantly positive correlation between caveolin-1 and the invasiveness of chorionicnoma cells (r=0.086,P<0.05);(3) CAV-1 siRNA could knock-out the expression of CAV-1 mRNA, and inhibit the invasion and proliferation ability of chorionicnoma cells.

CONCLUSION

CAV-1 can promote the invasion ability of chorionicnoma cells. CAV-1 siRNA can inhibit the invasion and proliferation ability of chorionicnoma cells.

目的

探讨小窝蛋白-1（CAV-1）表达与绒毛膜癌侵袭之间的关系，并探究CAV-1小干扰RNA（siRNA）对绒毛膜癌细胞系JEG-3侵袭能力的影响。

方法

（1）采用基质胶侵袭实验和3-(4,4)-二甲基噻唑-2,5-二苯基四氮唑溴盐（MTT）实验检测JEG-3细胞和JAR细胞侵袭与增殖能力的差异；（2）通过半定量逆转录聚合酶链反应（RT-PCR）检测人绒毛膜组织及绒毛膜癌细胞系（JEG-3细胞和JAR细胞）中小窝蛋白-1基因的表达。（3）采用RT-PCR、基质胶侵袭实验和MTT实验检测CAV-1 siRNA转染对CAV-1 mRNA表达以及JEG-3细胞侵袭与增殖能力的影响。

结果

（1）JEG-3细胞系的侵袭能力强于JAR细胞系（P<0.05），但JAR细胞与JEG-3细胞增殖能力的差异不明显（P>0.05）；（2）绒毛膜癌细胞系中小窝蛋白-1基因的表达明显强于正常人体绒毛（P<0.05），且JEG-3细胞中小窝蛋白-1基因的表达强于JAR细胞（P<0.05）。数据表明小窝蛋白-1与绒毛膜癌细胞的侵袭性之间存在显著正相关（r=0.086，P<0.05）；（3）CAV-1 siRNA可敲除CAV-1 mRNA的表达，并抑制绒毛膜癌细胞的侵袭与增殖能力。