白细胞介素-17对JEG-3人绒毛膜癌细胞增殖和侵袭的影响。
The effect of interleukin-17 on the proliferation and invasion of JEG-3 human choriocarcinoma cells.
作者信息
Pongcharoen Sutatip, Niumsup Pannika, Sanguansermsri Donruedee, Supalap Kwansuda, Butkhamchot Puntharee
机构信息
Department of Medicine, Faculty of Medicine, Naresuan University, Phitsanulok, Thailand.
出版信息
Am J Reprod Immunol. 2006 Apr;55(4):291-300. doi: 10.1111/j.1600-0897.2006.00366.x.
PROBLEM
As there has been a study in mice showing the expression of IL-17 by decidual cells and the status of IL-17 receptor expression in human pregnancy is not known, we hypothesized that IL-17 may regulate human trophoblast proliferation and invasion.
METHOD OF STUDY
JEG-3 cell line was used as a model for human trophoblast. Immunohistochemitry and reverse transcriptase polymerase chain reaction techniques were used to identify IL-17 receptor protein and mRNA, respectively. The effects of IL-17 on JEG-3 cell proliferation and invasion were tested using the BrdU incorporation and the Matrigel invasion assays, respectively.
RESULTS
IL-17 increased the invasive capacity of JEG-3 cells but had no effect on the proliferation and multinucleated formation of JEG-3 cells.
CONCLUSION
In this JEG-3 cell model of human trophoblast, the IL-17R and IL-17 may have a regulatory role in trophoblast invasion.
问题
由于有一项在小鼠身上进行的研究显示蜕膜细胞可表达白细胞介素-17（IL-17），而人类妊娠中IL-17受体的表达状况尚不清楚，我们推测IL-17可能调节人滋养层细胞的增殖和侵袭。
研究方法
采用JEG-3细胞系作为人滋养层细胞的模型。分别运用免疫组织化学和逆转录聚合酶链反应技术鉴定IL-17受体蛋白和信使核糖核酸（mRNA）。分别使用溴脱氧尿苷（BrdU）掺入法和基质胶侵袭试验检测IL-17对JEG-3细胞增殖和侵袭的影响。
结果
IL-17增强了JEG-3细胞的侵袭能力，但对JEG-3细胞的增殖和多核形成没有影响。
结论
在这个JEG-3人滋养层细胞模型中，IL-17受体（IL-17R）和IL-17可能在滋养层细胞侵袭中发挥调节作用。
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