与人类碳酸酐酶II复合的129Xe-隐色团生物传感器的结构
Structure of a 129Xe-cryptophane biosensor complexed with human carbonic anhydrase II.
作者信息
Aaron Julie A, Chambers Jennifer M, Jude Kevin M, Di Costanzo Luigi, Dmochowski Ivan J, Christianson David W
机构信息
Roy and Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6323, USA.
出版信息
J Am Chem Soc. 2008 Jun 4;130(22):6942-3. doi: 10.1021/ja802214x. Epub 2008 May 8.
Abstract
Cryptophanes represent an exciting class of xenon-encapsulating molecules that can be exploited as probes for nuclear magnetic resonance imaging. The 1.70 A resolution crystal structure of a cryptophane-derivatized benezenesulfonamide complexed with human carbonic anhydrase II shows how an encapsulated xenon atom can be directed to a specific biological target. The crystal structure confirms binding measurements indicating that the cryptophane cage does not strongly interact with the surface of the protein, which may enhance the sensitivity of 129Xe NMR spectroscopic measurements in solution.
摘要
穴番是一类令人兴奋的能够包封氙气的分子，可作为核磁共振成像的探针。一种穴番衍生的苯磺酰胺与人类碳酸酐酶II形成的配合物的晶体结构分辨率为1.70 Å，该结构展示了一个被包封的氙原子如何被导向一个特定的生物靶点。晶体结构证实了结合测量结果，表明穴番笼与蛋白质表面的相互作用不强，这可能会提高溶液中129Xe NMR光谱测量的灵敏度。
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