Busch F W, Mutter W, Koszinowski U H, Reddehase M J
Medical University Clinic, Tübingen, Federal Republic of Germany.
J Virol. 1991 Feb;65(2):981-4. doi: 10.1128/JVI.65.2.981-984.1991.
The effect of murine cytomegalovirus on myelopoiesis was studied in long-term bone marrow culture to find an in vitro correlate for the lethal virus interference with bone marrow reconstitution (W. Mutter, M. J. Reddehase, F. W. Busch, H.-J. Bühring, and U. H. Koszinowski, J. Exp. Med. 167:1645-1658, 1988). The in vitro generation of granulocyte-monocyte progenitors (CFU-GM) discontinued after infection of the stromal cell layer, whereas the proliferation and differentiation of CFU-GM to granulocyte-monocyte colonies remained unaffected. A protocol was established to probe the functional integrity of earlier hematopoietic cells. Pre-CFU-GM (the progenitors of the CFU-GM) could be recovered from an infected bone marrow donor culture by transfer onto an inductive recipient stromal cell layer. Thus, at least in vitro, infection of bone marrow stroma appears to be the only cause of the defect in myelopoiesis.
为了找到致死性病毒干扰骨髓重建的体外相关因素，在长期骨髓培养中研究了鼠巨细胞病毒对骨髓生成的影响（W. Mutter、M. J. Reddehase、F. W. Busch、H.-J. Bühring和U. H. Koszinowski，《实验医学杂志》167:1645 - 1658，1988年）。基质细胞层感染后，粒细胞 - 单核细胞祖细胞（CFU - GM）的体外生成停止，而CFU - GM向粒细胞 - 单核细胞集落的增殖和分化仍未受影响。建立了一个方案来检测早期造血细胞的功能完整性。通过转移到诱导性受体基质细胞层，可以从感染的骨髓供体培养物中回收前CFU - GM（CFU - GM的祖细胞）。因此，至少在体外，骨髓基质感染似乎是骨髓生成缺陷的唯一原因。
