Preiksaitis J K, Janowska-Wieczorek A
Department of Medicine, University of Alberta, Edmonton, Canada.
J Med Virol. 1991 Oct;35(2):76-84. doi: 10.1002/jmv.1890350203.
Using pre-established human long-term marrow culture (LTMC), we studied cytomegalovirus (CMV) replication in this system after in vitro infection of nonadherent cells obtained from these cultures with CMV AD-169. After infection with 5 immediate-early antigen foci/cell CMV was detectable for 63-123 days (peak titer 2.0 x 10(3)-1.3 x 10(9)) in the supernatants of LTMC. Lower MOI resulted in a delay in the detection and longer persistence of CMV in LTMC although peak titers were unchanged. CMV infection was associated with destruction of the stromal layer, appearance of a subset of large (23 microns) CMV-infected mononuclear cells in the nonadherent fraction, and early differentiation of nonadherent cells into a homogenous population of macrophage-like cells. CMV infection resulted in a reduction and premature disappearance of committed progenitors (BFU-E, CFU-GM) in LTMC. Persistence of CMV in LTMC was linked to ongoing hemopoiesis. Human bone marrow may be an important site for CMV replication during acute infection and CMV persistence.
利用预先建立的人长期骨髓培养（LTMC）体系，我们在用巨细胞病毒（CMV）AD - 169体外感染从这些培养物中获得的非贴壁细胞后，研究了CMV在该体系中的复制情况。在用5个即刻早期抗原灶/细胞进行感染后，在LTMC的上清液中，CMV可检测到63 - 123天（峰值滴度为2.0×10³ - 1.3×10⁹）。较低的感染复数（MOI）导致CMV在LTMC中的检测延迟且持续时间更长，尽管峰值滴度不变。CMV感染与基质层的破坏、非贴壁部分中出现一部分大的（23微米）被CMV感染的单核细胞以及非贴壁细胞早期分化为均匀的巨噬细胞样细胞群体有关。CMV感染导致LTMC中定向祖细胞（BFU - E、CFU - GM）数量减少和过早消失。CMV在LTMC中的持续存在与持续的造血作用有关。人骨髓可能是急性感染和CMV持续存在期间CMV复制的重要部位。
