Honda Z, Nakamura M, Miki I, Minami M, Watanabe T, Seyama Y, Okado H, Toh H, Ito K, Miyamoto T
Department of Physiological Chemistry and Nutrition, Faculty of Medicine, University of Tokyo, Japan.
Nature. 1991 Jan 24;349(6307):342-6. doi: 10.1038/349342a0.
Platelet-activating factor (PAF), a unique phospholipid mediator, possesses potent proinflammatory, smooth-muscle contractile and hypotensive activities, and appears to be crucial in the pathogenesis of bronchial asthma and in the lethality of endotoxin and anaphylactic shock. Despite this, little is known of the molecular properties of the PAF receptor and related signal transduction systems. Although several lines of evidence suggest that activation of the PAF receptor stimulates phospholipase C and subsequent inositol trisphosphate formation through G protein(s), the PAF receptor and calcium channel are reported to show a close relation. As a first approach to cloning lipid autacoid receptors, we have isolated complementary DNA for the PAF receptors. Our strategy involved gene expression in Xenopus laevis oocytes and electrophysiological detection of PAF-induced responses. Sequence analysis indicates that the receptor belongs to the superfamily of G protein-coupled receptors.
血小板活化因子（PAF）是一种独特的磷脂介质，具有强大的促炎、平滑肌收缩和降压活性，在支气管哮喘的发病机制以及内毒素和过敏性休克的致死性方面似乎起着关键作用。尽管如此，关于PAF受体的分子特性及相关信号转导系统却知之甚少。虽然有几条证据表明PAF受体的激活通过G蛋白刺激磷脂酶C及随后的肌醇三磷酸形成，但据报道PAF受体与钙通道关系密切。作为克隆脂质自分泌受体的第一步，我们已分离出PAF受体的互补DNA。我们的策略包括在非洲爪蟾卵母细胞中进行基因表达以及对PAF诱导反应进行电生理检测。序列分析表明该受体属于G蛋白偶联受体超家族。
