Feyles V, Sikora L K, McGarry R C, Jerry L M
Oncology Research Group, University of Calgary, Alta., Canada.
Oncology. 1991;48(1):58-64. doi: 10.1159/000226896.
The dispersed neuroendocrine system includes cells with different embryological derivations, sharing a common neuroendocrine (NE) program, as indicated by the expression of NE markers, some of which are shared antigenic determinants. We report here that the small cell lung carcinoma cells NCI-H69 express the two human melanoma-associated antigens (HMAA) NGA/LS62 an LS109. Incubation of NCI-H69 cells with maturational inducers, such as retinoic acid and bromodeoxyuridine (BrdU), upregulated the expression of both HMAA. Exposure to BrdU for 4 weeks induced the appearance of a different phenotype in subpopulations of NCI-H69 cells, which became epithelioid, substrate-adherent, grew in monolayer and continued to express NE-associated antigens in variable amount. The shift in phenotype was not reversible after BrdU withdrawal and was maintained for at least 6 months in continuous culture. The substrate adhesion of NCI-H69 cells was paralleled by a change in NGA glycosylation pattern, thus suggesting a possible functional role for NGA in cell substrate adhesion/recognition.
弥散神经内分泌系统包括具有不同胚胎学来源的细胞，这些细胞共享一个共同的神经内分泌（NE）程序，这由NE标志物的表达所表明，其中一些是共同的抗原决定簇。我们在此报告，小细胞肺癌细胞NCI-H69表达两种人类黑色素瘤相关抗原（HMAA）NGA/LS62和LS109。用成熟诱导剂如视黄酸和溴脱氧尿苷（BrdU）孵育NCI-H69细胞，上调了两种HMAA的表达。用BrdU处理4周诱导NCI-H69细胞亚群中出现不同的表型，这些细胞变成上皮样、贴壁于底物、单层生长并继续以不同量表达NE相关抗原。BrdU撤除后表型转变不可逆，并且在连续培养中至少维持6个月。NCI-H69细胞的底物黏附与NGA糖基化模式的变化平行，因此提示NGA在细胞底物黏附/识别中可能具有功能作用。
