Feyles V, Dixon W T, Sikora L K, McGarry R C, Jerry L M
Oncology Research Group, University of Calgary, Alberta, Canada.
Cancer Immunol Immunother. 1991;32(5):261-72. doi: 10.1007/BF01789043.
We have described two human melanoma-associated antigens (HMAA), recognized by the murine monoclonal antibodies LS62 and LS109. LS62 recognizes the neuroglandular antigen (NGA), which is overexpressed in neoplastic melanocytes as well as in several tissues of neuroectodermal origin. These antibodies were used to screen six neuroblastoma cell lines and one neuroepithelioma cell line. A melanoma cell line, G361, known to express the two antigens, was used as the positive control. Variable expression of the two antigens was detected in neuroblastoma cells. The surface expression of NGA and of the LS109 antigen was modulated in parallel with the morphological differentiation induced by retinoic acid, 5-bromodeoxyuridine, or cyclic AMP analog/activators. The modulation of the expression of the two HMAA was detected in G361 melanoma cells and in one of the neuroblastoma cell lines, SK-N-SH. These results suggest altered expression of both antigens during melanoma and neuroblastoma cell differentiation in culture.
我们已经描述了两种人类黑色素瘤相关抗原（HMAA），它们可被鼠单克隆抗体LS62和LS109识别。LS62识别神经腺抗原（NGA），该抗原在肿瘤性黑素细胞以及几种神经外胚层起源的组织中过度表达。这些抗体被用于筛选六种神经母细胞瘤细胞系和一种神经上皮瘤细胞系。已知表达这两种抗原的黑色素瘤细胞系G361用作阳性对照。在神经母细胞瘤细胞中检测到这两种抗原的可变表达。NGA和LS109抗原的表面表达与视黄酸、5-溴脱氧尿苷或环磷酸腺苷类似物/激活剂诱导的形态分化平行调节。在G361黑色素瘤细胞和一种神经母细胞瘤细胞系SK-N-SH中检测到两种HMAA表达的调节。这些结果表明，在培养的黑色素瘤和神经母细胞瘤细胞分化过程中，两种抗原的表达均发生了改变。
