在分离的豚鼠和大鼠肝线粒体中存在环磷酸腺苷依赖性蛋白激酶活性的证据。
Evidence for cyclic AMP-dependent protein kinase activity in isolated guinea pig and rat liver mitochondria.
作者信息
Kleitke B, Sydow H, Wollenberger A
出版信息
Acta Biol Med Ger. 1976;35(3-4):K9-K17.
PMID:184629
Abstract
Purified preparations of guinea pig and rat liver mitochondria contain considerable latent cAMP-dependent protein kinase activity that is revealed by treatment with 1% Triton X-100. The solubilized kinase was partly purified by DEAE-cellulose chromatography. It accepts protein in the washed Triton-extracted mitochondria as substrate.
摘要
豚鼠和大鼠肝脏线粒体的纯化制剂含有相当数量的潜在环磷酸腺苷（cAMP）依赖性蛋白激酶活性，用1% Triton X-100处理可将其激活。通过DEAE-纤维素色谱法对溶解的激酶进行了部分纯化。它可将经洗涤的Triton提取物处理过的线粒体中的蛋白质作为底物。
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