Pelletier I, Couderc T, Borzakian S, Wyckoff E, Crainic R, Ehrenfeld E, Colbere-Garapin F
Unité de Virologie Médicale, Institut Pasteur, Paris, France.
Virology. 1991 Feb;180(2):729-37. doi: 10.1016/0042-6822(91)90086-q.
Six Sabin-derived persistent poliovirus mutants were selected in human neuroblastoma IMR-32 cells. The mutants had a titer 30 to 10(5) times lower in nonneural HEp-2c cells than in IMR-32 cells. When the growth cycles of persistent viruses in the two cell lines were compared, the most striking feature was a delay of 2 to 4 hr in virus release from HEp-2c cells. In Hep-2c cells, type 1 mutants could spontaneously establish a persistent infection in the absence of any exogenous viral inhibitor. Mutations at a rate of 1 every 210 nucleotides had accumulated in the genome of the type 1 mutants selected in neuroblastoma cells, modifying cell specificity and conferring the ability to persist in some nonneural cells. These results indicate that mutants of poliovirus with highly modified biological properties can be selected in vitro in cells of neural origin.
在人神经母细胞瘤IMR-32细胞中筛选出6种源自萨宾株的持续性脊髓灰质炎病毒突变体。这些突变体在非神经HEp-2c细胞中的滴度比在IMR-32细胞中低30至10⁵倍。当比较这两种细胞系中持续性病毒的生长周期时，最显著的特征是病毒从HEp-2c细胞释放延迟2至4小时。在HEp-2c细胞中，1型突变体在没有任何外源性病毒抑制剂的情况下能够自发建立持续性感染。在神经母细胞瘤细胞中筛选出的1型突变体基因组中每210个核苷酸积累1个突变，改变了细胞特异性并赋予了在一些非神经细胞中持续存在的能力。这些结果表明，具有高度改变生物学特性的脊髓灰质炎病毒突变体可以在神经源性细胞中体外筛选出来。
