鉴定脊髓灰质炎病毒基因组中与HEp-2细胞持续感染有关的区域。
Identification of a region of the poliovirus genome involved in persistent infection of HEp-2 cells.
作者信息
Calvez V, Pelletier I, Borzakian S, Colbère-Garapin F
机构信息
Unité de Virologie Médicale, Institute Pasteur, Paris, France.
出版信息
J Virol. 1993 Jul;67(7):4432-5. doi: 10.1128/JVI.67.7.4432-4435.1993.
Abstract
Poliovirus mutants were selected during the persistent infection of human neuroblastoma cells. These viruses could establish secondary persistent infections in HEp-2 nonneural cells. We report the identification of a region of the genome of a persistent virus (S11) that was sufficient to confer to a recombinant virus the phenotype that causes persistent infection in HEp-2 cells. This region, between nucleotides 1148 and 3481, contained 11 missense mutations mapping exclusively in the genes of capsid proteins VP1 and VP2. Because recombinant viruses carrying only one of these two mutated genes were not able to cause persistent infection, it seems very probable that two or more mutations in these genes are required for expression of the phenotype that causes persistent infection.
摘要
脊髓灰质炎病毒突变体是在人神经母细胞瘤细胞的持续感染过程中筛选出来的。这些病毒能够在HEp-2非神经细胞中建立继发性持续感染。我们报告了一种持续病毒（S11）基因组区域的鉴定结果，该区域足以赋予重组病毒在HEp-2细胞中引起持续感染的表型。这个位于核苷酸1148至3481之间的区域包含11个错义突变，这些突变仅定位在衣壳蛋白VP1和VP2的基因中。由于仅携带这两个突变基因之一的重组病毒无法引起持续感染，因此很可能这些基因中的两个或更多个突变是表达引起持续感染的表型所必需的。
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