Ruike Yoshinao, Ichimura Atsuhiko, Tsuchiya Soken, Shimizu Kazuharu, Kunimoto Ryo, Okuno Yasushi, Tsujimoto Gozoh
Department of Genomic Drug Discovery Science, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida Shimoadachi-cho Sakyo-ku, Kyoto, 606-8501, Japan.
Department of Nanobio Drug Discovery, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.
J Hum Genet. 2008;53(6):515. doi: 10.1007/s10038-008-0279-x. Epub 2008 May 10.
Microribonucleic acids (miRNAs) are small noncoding RNAs that negatively regulate gene expression at the posttranscriptional level. Although considerable progress has been made in studying the function of miRNAs, they still remain largely unclear, mainly because of the difficulty in identifying target genes for miRNA. We performed a global analysis of both miRNAs and mRNAs expression across 16 human cell lines and extracted negatively correlated pairs of miRNA and mRNA which indicate miRNA-target relationship. The many of known-target of miR-124a showed negative correlation, suggesting our analysis were valid. We further extracted physically relevant miRNA-target gene pairs, applying computational target prediction algorithm with inverse correlations of miRNA and messenger RNA (mRNA) expression. Furthermore, gene-ontology-based annotation and functional enrichment analysis of the extracted miRNA-target gene pairs made it possible to indicate putative functions of miRNAs. The data collected here will be of value for further studies into the function of miRNA.
微小核糖核酸（miRNA）是一类小的非编码RNA，它们在转录后水平上对基因表达进行负调控。尽管在研究miRNA功能方面已经取得了相当大的进展，但它们在很大程度上仍不清楚，主要是因为难以鉴定miRNA的靶基因。我们对16种人类细胞系中的miRNA和mRNA表达进行了全面分析，并提取了表明miRNA-靶标关系的负相关miRNA和mRNA对。miR-124a的许多已知靶标显示出负相关性，表明我们的分析是有效的。我们进一步应用miRNA与信使RNA（mRNA）表达呈负相关的计算靶标预测算法，提取了物理相关的miRNA-靶标基因对。此外，基于基因本体的注释和对提取的miRNA-靶标基因对的功能富集分析，使得指明miRNA的推定功能成为可能。这里收集的数据将对进一步研究miRNA的功能具有价值。
