Giaginis Constantinos, Georgiadou Maria, Dimakopoulou Konstantina, Tsourouflis Gerasimos, Gatzidou Elisavet, Kouraklis Gregorios, Theocharis Stamatios
Department of Forensic Medicine and Toxicology, Medical School, University of Athens, 75 M. Asias str, Goudi, Athens, 11527, Greece.
Dig Dis Sci. 2009 Feb;54(2):282-91. doi: 10.1007/s10620-008-0305-z. Epub 2008 May 9.
Minichromosome maintenance (MCM) proteins are essential components of DNA replication, being related to cell proliferation, and serve as useful markers for cancer screening, surveillance, and prognosis. Our aim was to examine the clinical significance of MCM-2 and MCM-5 protein expression in colon cancer and to evaluate the association with various clinicopathological characteristics and tumor proliferative capacity. Immunohistochemical expression of MCM-2 and MCM-5 was performed on paraffin-embedded malignant tissue sections obtained from 96 patients with colon cancer. MCM-2 and MCM-5 expression was correlated with different clinicopathological characteristics, proliferative capacity (Ki-67 labeling index), and p53 cell-cycle regulator expression. MCM-2 and Ki-67 expression was significantly associated with the tumors' histological grade (P = 0.003), existence of nodular metastases (N) (P = 0.003 and P = 0.030, respectively), malignancy on adenoma (P = 0.029 and P = 0.024, respectively), and vascular invasion (P = 0.010 and P = 0.011, respectively). MCM-2 expression was additionally associated with Dukes' stage (P = 0.005). Significant positive relationships were found between the expression of MCM-2 or MCM-5 proteins and that of Ki-67 protein (r = 0.963, P-value < 0.001, and r = 0.738, P-value < 0.001, respectively), as well as between MCM-2 and MCM-5 proteins (r = 0.745, P-value < 0.001). Significant positive relationships were also observed between the expression of MCM-2 or MCM-5 proteins and that of p53 protein; however, they were consistently lower than the corresponding with Ki-67 protein. No significant association was observed between MCM-5 protein expression and the clinicopathological characteristics examined. The current data suggest that MCM-2 protein expression is significantly associated with important clinicopathological characteristics for patients' management, being correlated with the cell proliferation state in colon cancer.
微小染色体维持（MCM）蛋白是DNA复制的重要组成部分，与细胞增殖相关，可作为癌症筛查、监测及预后的有用标志物。我们的目的是研究MCM-2和MCM-5蛋白表达在结肠癌中的临床意义，并评估其与各种临床病理特征及肿瘤增殖能力的相关性。对96例结肠癌患者石蜡包埋的恶性组织切片进行MCM-2和MCM-5的免疫组化表达检测。MCM-2和MCM-5的表达与不同的临床病理特征、增殖能力（Ki-67标记指数）及p53细胞周期调节因子表达相关。MCM-2和Ki-67的表达与肿瘤的组织学分级（P = 0.003）、结节性转移（N）的存在（分别为P = 0.003和P = 0.030）、腺瘤恶变（分别为P = 0.029和P = 0.024）以及血管侵犯（分别为P = 0.010和P = 0.011）显著相关。MCM-2的表达还与Dukes分期相关（P = 0.005）。MCM-2或MCM-5蛋白的表达与Ki-67蛋白的表达之间存在显著正相关（r = 0.963，P值< 0.001；r = 0.738，P值< 0.001），MCM-2和MCM-5蛋白之间也存在显著正相关（r = 0.745，P值< 0.001）。MCM-2或MCM-5蛋白的表达与p53蛋白的表达之间也观察到显著正相关；然而，它们始终低于与Ki-67蛋白的相应相关性。未观察到MCM-5蛋白表达与所检测的临床病理特征之间存在显著关联。目前的数据表明，MCM-2蛋白表达与患者管理的重要临床病理特征显著相关，与结肠癌中的细胞增殖状态相关。
