Wojnar Andrzej, Kobierzycki Christopher, Krolicka Anna, Pula Bartosz, Podhorska-Okolow Marzena, Dziegiel Piotr
Lower Silesian Oncology Centre, Wroclaw, Poland.
Folia Histochem Cytobiol. 2010 Sep 30;48(3):442-6. doi: 10.2478/v10042-010-0069-0.
The study aimed at examining a relationship between expression of Ki-67 antigen and minichromosome maintenance 2 protein (MCM-2) and a grade of histological malignancy G in ductal breast cancers. The function of widely used marker of proliferation Ki-67 is still not clear. In contrast, the MCM-2 protein is well known to play an important role in controlling the cell cycle. Both proteins represent small protein molecules, which manifest nuclear expression only during cell division of normal and neoplastic cells. Their expression is noted in several malignant tumours. These studies were conducted on 56 archival paraffin blocks of ductal breast cancers. Immunohistochemical reactions were performed using monoclonal Ki-67- and MCM-2-specific antibodies. Statistical analysis demonstrated a positive correlation between expressions of two proteins (r=0.6; p<0.05). The most intense expression of these two markers was demonstrated in G3 grade cancers. Statistical analysis showed more pronounced expression of Ki-67 antigen in G3 grade cancers as compared to cancers of G1 and G2 grades (p<0.001) and, in the case of MCM-2 protein, a more pronounced expression in G3 grade cancers, as compared to those of G1 (p<0.05) or G2 grade (p<0.01). The results obtained in our study suggest that MCM-2 could be used as a marker of proliferation in breast carcinomas.
该研究旨在探讨乳腺导管癌中Ki-67抗原与微小染色体维持蛋白2（MCM-2）的表达与组织学恶性程度分级G之间的关系。广泛应用的增殖标志物Ki-67的功能仍不明确。相比之下，MCM-2蛋白在控制细胞周期中起着重要作用，这一点广为人知。这两种蛋白均为小分子蛋白，仅在正常细胞和肿瘤细胞的细胞分裂过程中呈核表达。在几种恶性肿瘤中均观察到它们的表达。本研究对56例乳腺导管癌存档石蜡块进行。使用单克隆Ki-67特异性抗体和MCM-2特异性抗体进行免疫组化反应。统计分析显示两种蛋白的表达之间呈正相关（r = 0.6；p < 0.05）。这两种标志物的最强表达见于G3级癌症。统计分析表明，与G1级和G2级癌症相比，Ki-67抗原在G3级癌症中的表达更明显（p < 0.001）；就MCM-2蛋白而言，与G1级癌症（p < 0.05）或G2级癌症（p < 0.01）相比，其在G3级癌症中的表达更明显。我们的研究结果表明，MCM-2可作为乳腺癌增殖的标志物。
