Bottaro D P, Rubin J S, Faletto D L, Chan A M, Kmiecik T E, Vande Woude G F, Aaronson S A
Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD 20892.
Science. 1991 Feb 15;251(4995):802-4. doi: 10.1126/science.1846706.
Hepatocyte growth factor (HGF) is a plasminogen-like protein thought to be a humoral mediator of liver regeneration. A 145-kilodalton tyrosyl phosphoprotein observed in rapid response to HGF treatment of intact target cells was identified by immunoblot analysis as the beta subunit of the c-met proto-oncogene product, a membrane-spanning tyrosine kinase. Covalent cross-linking of 125I-labeled ligand to cellular proteins of appropriate size that were recognized by antibodies to c-met directly established the c-met product as the cell-surface receptor for HGF.
肝细胞生长因子（HGF）是一种类似纤溶酶原的蛋白质，被认为是肝脏再生的体液介质。通过免疫印迹分析确定，在完整靶细胞经HGF处理后迅速出现的一种145千道尔顿的酪氨酰磷酸化蛋白是原癌基因c-met产物的β亚基，c-met是一种跨膜酪氨酸激酶。将125I标记的配体与能被c-met抗体识别的合适大小的细胞蛋白进行共价交联，直接证实了c-met产物是HGF的细胞表面受体。
