Dich J, Gluud C N
Acta Physiol Scand. 1976 Aug;97(4):457-69. doi: 10.1111/j.1748-1716.1976.tb10285.x.
Parenchymal rat liver cells were isolated by the collagenase method and incubated in Krebs-Henseleit buffer containing 0.5% gelatin. The basal level of cyclic AMP in isolated cells was 0.52 nmol per g liver wet wt. Glucagon (10(-10)-10(-6) M) caused a significant increase in the level of cyclic AMP. Maximum levels were obtained 2-15 min after addition of glucagon. Repeated administration of glucagon caused a new increase in cyclic AMP, but the response was lesser than after the first addition of glucagon, indicating refractoriness to glucagon. The rate of albumin secretion was 4.6 mug/min per g liver wet wt. This is about the rate found in the perfused liver, Glucagon (10(-8-10(-6) M) inhibited albumin secretion and the incorporation of 14C-leucine into albumin, into total proteins in the medium and into total proteins in the cell suspension. The effect of glucagon on albumin secretion is compatible with an effect on the rate of synthesis. A positive correlation existed between the maximal level of cyclic AMP after glucagon administration and the inhibition of both albumin secretion and the incorporation of 149leucine.
采用胶原酶法分离大鼠肝实质细胞，并在含有0.5%明胶的Krebs-Henseleit缓冲液中孵育。分离细胞中环磷酸腺苷（cAMP）的基础水平为每克肝脏湿重0.52纳摩尔。胰高血糖素（10⁻¹⁰ - 10⁻⁶ M）可使cAMP水平显著升高。加入胰高血糖素后2 - 15分钟达到最高水平。重复给予胰高血糖素会使cAMP再次升高，但反应程度小于首次加入胰高血糖素后，表明对胰高血糖素产生了不应性。白蛋白分泌速率为每克肝脏湿重4.6微克/分钟。这与灌注肝脏中的速率相近。胰高血糖素（10⁻⁸ - 10⁻⁶ M）抑制白蛋白分泌以及14C-亮氨酸掺入白蛋白、培养基中的总蛋白和细胞悬液中的总蛋白。胰高血糖素对白蛋白分泌的影响与对合成速率的影响一致。给予胰高血糖素后cAMP的最高水平与白蛋白分泌抑制以及14C-亮氨酸掺入抑制之间存在正相关。
