traY和traI是oriT依赖性增强含lac质粒与λplac5之间重组所必需的。
traY and traI are required for oriT-dependent enhanced recombination between lac-containing plasmids and lambda plac5.
作者信息
Carter J R, Porter R D
机构信息
Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
出版信息
J Bacteriol. 1991 Feb;173(3):1027-34. doi: 10.1128/jb.173.3.1027-1034.1991.
Abstract
Recombination between F42lac and lambda plac5 is typically 20- to 50-fold more efficient than recombination between chromosomal lac and lambda plac5. This enhancement of recombination requires trans-acting factors located in the promoter-distal and promoter-proximal regions of the main traY-to-traI (traZ) operon. By testing the ability of deletion mutants of tra to support enhanced recombination, we have identified traY as the only product has been ruled out. We also report that traI is the only gene from the promoter-distal end of the traY to traI operon that is required for recombination enhancement. Of the two proposed domains of traI, we conclude that the oriT-nicking activity is essential, whereas the helicase activity is largely dispensable. The possibility of a third traI activity is also discussed.
摘要
F42lac与λplac5之间的重组效率通常比染色体lac与λplac5之间的重组效率高20至50倍。这种重组增强需要位于主traY至traI（traZ）操纵子启动子远端和启动子近端区域的反式作用因子。通过测试tra缺失突变体支持增强重组的能力，我们已确定traY是唯一被排除的产物。我们还报告说，traI是traY至traI操纵子启动子远端末端唯一需要增强重组的基因。在traI的两个假定结构域中，我们得出结论，oriT切口活性是必不可少的，而解旋酶活性在很大程度上是可有可无的。还讨论了traI第三种活性的可能性。
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