Seifert H S, Porter R D
Mol Gen Genet. 1984;193(2):269-74. doi: 10.1007/BF00330679.
F42lac recombination with lambda plac is normally 20-fold to 50-fold higher than recombination between lambda plac and a chromosomal lac gene. Transductional crosses with lambda plac and a recombinant plasmid containing the lac operon and F replication functions show only two to four-fold higher recombination than similar crosses with a chromosomal lac gene. Insertion of a BamHI fragment containing the entire tra regulon of F into the mini-F-lac plasmid restores the high level of recombination seen with F42lac.
F42lac与λplac的重组通常比λplac与染色体lac基因之间的重组高20至50倍。用λplac和含有lac操纵子及F复制功能的重组质粒进行的转导杂交，其重组率仅比用染色体lac基因进行的类似杂交高2至4倍。将含有F完整tra调控子的BamHI片段插入mini-F-lac质粒，可恢复F42lac所见的高水平重组。
