recB突变对大肠杆菌HfrH菌株的特定影响。
Specific effects of a recB mutation on the HfrH strain of Escherichia coli.
作者信息
Dermić D, Trgovcević Z
机构信息
Ruer Bosković Institute, 10001 Zagreb, Croatia.
出版信息
J Bacteriol. 1999 Feb;181(4):1334-7. doi: 10.1128/JB.181.4.1334-1337.1999.
Abstract
The recB268::Tn10 mutation was introduced into the HfrH strain of Escherichia coli. Compared with recB F- and recB F+ cells, the viability of this mutant strain was much lower. Compared with wild-type HfrH, the recB derivative donated much shorter fragments of its chromosome to the recipient. It is suggested that the recB gene product (i.e., RecBCD enzyme) participates in Hfr transfer.
摘要
recB268::Tn10突变被引入大肠杆菌的HfrH菌株中。与recB F-和recB F+细胞相比，该突变菌株的活力要低得多。与野生型HfrH相比，recB衍生物向受体捐赠的染色体片段要短得多。有人提出recB基因产物（即RecBCD酶）参与Hfr转移。
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