岩蔷薇茶对原位牙菌斑细菌定植及酶活性的影响。

Effects of Cistus-tea on bacterial colonization and enzyme activities of the in situ pellicle.

作者信息

Hannig Christian, Spitzmüller Bettina, Al-Ahmad Ali, Hannig Matthias

机构信息

Department of Operative Dentistry and Periodontology, University of Freiburg, Hugstetter Strasse 55, 79106 Freiburg, Germany.

出版信息

J Dent. 2008 Jul;36(7):540-5. doi: 10.1016/j.jdent.2008.04.002. Epub 2008 May 12.

DOI:10.1016/j.jdent.2008.04.002

PMID:18468764

Abstract

OBJECTIVES

Polyphenols are expected to have antibacterial properties. Cistus is a tea rich in polyphenols. The aim of the present in situ study was to investigate the effect of Cistus-tea on the pellicle and on the initial oral biofilm.

METHODS

For in situ pellicle formation and initial biofilm formation, bovine enamel slabs were fixed on maxillary splints and carried by four subjects at buccal sites for up to 2 h. Bacteria present in 120-min pellicles were determined with DAPI-staining and fluorescence in situ hybridization with and without a 10 min rinse with Cistus-tea performed 1 min after incorporation of the slabs. In addition, amylase, lysozyme, glucosyltransferase and peroxidase activities immobilised in the pellicle layer were measured before and after rinsing for 10 min with Cistus-tea.

RESULTS

The amount of bacteria detected in the 120-min biofilm was reduced significantly, if a 10 min rinse with Cistus-tea was performed one min after insertion of the enamel slabs. DAPI-staining yielded 13.2+/-3.5 for controls and 6.5+/-1.1 x 10(4) bacteria/cm(2), if a rinse with Cistus-tea was applied. Lysozyme, amylase and glucosyltransferase activities immobilised in the pellicle were not affected following a rinse with Cistus-tea. However, peroxidase activity was reduced significantly.

CONCLUSIONS

Cistus-tea may be used to reduce the initial bacterial adhesion in the oral cavity.

摘要

目的

多酚有望具有抗菌特性。岩蔷薇茶富含多酚。本原位研究的目的是调查岩蔷薇茶对牙菌斑和初始口腔生物膜的影响。

方法

为进行原位牙菌斑形成和初始生物膜形成实验，将牛牙釉质片固定在上颌夹板上，由四名受试者佩戴在颊侧部位长达2小时。在牙釉质片植入1分钟后，用岩蔷薇茶冲洗10分钟，然后通过4′,6-二脒基-2-苯基吲哚（DAPI）染色以及荧光原位杂交法测定120分钟牙菌斑中的细菌。此外，在用岩蔷薇茶冲洗10分钟前后，测量固定在牙菌斑层中的淀粉酶、溶菌酶、葡糖基转移酶和过氧化物酶的活性。

结果

如果在牙釉质片插入1分钟后用岩蔷薇茶冲洗10分钟，120分钟生物膜中检测到的细菌数量会显著减少。进行岩蔷薇茶冲洗时，DAPI染色显示对照组每平方厘米有13.2±3.5个细菌，而处理组为6.5±1.1×10⁴个细菌/平方厘米。用岩蔷薇茶冲洗后，固定在牙菌斑中的溶菌酶、淀粉酶和葡糖基转移酶活性未受影响。然而，过氧化物酶活性显著降低。