常规漱口液对牙釉质和牙本质原位初始生物黏附的影响。

Effect of conventional mouthrinses on initial bioadhesion to enamel and dentin in situ.

机构信息

Clinic of Operative Dentistry, Medical Faculty Carl Gustav Carus, TU Dresden, Dresden, Germany.

出版信息

Caries Res. 2013;47(2):150-61. doi: 10.1159/000345083. Epub 2012 Nov 28.

DOI:10.1159/000345083

PMID:23207875

Abstract

AIM

The study aimed to investigate the effect of a customary fluoride solution, containing sodium fluoride and amine fluoride, on initial biofilm formation on enamel and dentin in situ compared directly to chlorhexidine.

METHODS

Bovine enamel and dentin specimens were mounted on maxillary splints carried by 9 subjects. After 1 min of pellicle formation, rinses with tap water (control), chlorhexidine (meridol med CHX 0.2%, GABA) and a fluoride mouthrinse (elmex, GABA) were performed for 1 min. Subsequently, the slabs were carried for another 8 h. The adherent bacteria were determined by DAPI staining, live-dead staining and determination of colony-forming units after desorption; glucan formation was visualized with concanavalin A. Additionally, energy-dispersive X-ray spectroscopy (EDX) analysis of the in situ biofilm layers was conducted, and contact angle measurements were performed. Statistical evaluation was performed by means of the Kruskal-Wallis test followed by the Mann-Whitney U test (p < 0.05).

RESULTS

In the control group, significantly higher amounts of adherent bacteria were detected on dentin (4.8 × 10(6) ± 5.4 × 10(6) bacteria/cm(2)) than on enamel (1.2 × 10(6) ± 1.5 × 10(6) bacteria/cm(2), DAPI). Chlorhexidine significantly reduced the amount of adherent bacteria (dentin: 2.8 × 10(5) ± 3.4 × 10(5) bacteria/cm(2); enamel: 4.2 × 10(5) ± 8.7 × 10(5) bacteria/cm(2)). Rinses with the fluoride solution also significantly reduced bacterial adherence to dentin (8.1 × 10(5) ± 1.5 × 10(6) bacteria/cm(2)). Fluoride could not be detected by EDX analysis of the biofilms. Fluoride mouthrinsing did not influence the wettability of the pellicle-covered enamel surface.

CONCLUSION

In addition to the reduction of demineralization and antibacterial effects, fluorides inhibit initial biofilm formation on dental hard tissues considerably, especially on dentin.

摘要

目的

本研究旨在直接比较含氟化物溶液（包含氟化钠和胺氟化物）与洗必泰对牙釉质和牙本质初始生物膜形成的影响。

方法

将牛牙釉质和牙本质标本固定在上颌夹板上，由 9 名受试者佩戴。在形成 1 分钟的黏膜后，用自来水（对照）、洗必泰（meridol med CHX 0.2%，GABA）和含氟漱口水（elmex，GABA）冲洗 1 分钟。然后，将牙夹板再佩戴 8 小时。通过 DAPI 染色、活/死染色和洗脱后菌落形成单位测定来确定附着的细菌；用 Concanavalin A 可视化葡聚糖形成。此外，还进行了原位生物膜层的能量色散 X 射线光谱（EDX）分析，并进行了接触角测量。采用 Kruskal-Wallis 检验 followed by the Mann-Whitney U 检验（p < 0.05）进行统计评估。

结果

在对照组中，牙本质上附着的细菌数量明显高于牙釉质（牙本质：4.8×10^6±5.4×10^6 个细菌/cm^2；牙釉质：1.2×10^6±1.5×10^6 个细菌/cm^2，DAPI）。洗必泰显著减少了附着的细菌数量（牙本质：2.8×10^5±3.4×10^5 个细菌/cm^2；牙釉质：4.2×10^5±8.7×10^5 个细菌/cm^2）。含氟溶液冲洗也显著减少了牙本质上的细菌附着（8.1×10^5±1.5×10^6 个细菌/cm^2）。EDX 分析未能检测到生物膜中的氟化物。氟化物漱口不会影响包被有黏膜的牙釉质表面的润湿性。