Hertel Susann, Graffy Leif, Pötschke Sandra, Basche Sabine, Al-Ahmad Ali, Hoth-Hannig Wiebke, Hannig Matthias, Hannig Christian
Clinic of Operative and Pediatric Dentistry, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Fetscherstraße 74, D-01307 Dresden, Germany.
Department of Operative Dentistry and Periodontology, Center for Dental Medicine, Medical Center, University of Freiburg, D-79106 Freiburg, Germany.
Arch Oral Biol. 2016 Nov;71:87-96. doi: 10.1016/j.archoralbio.2016.07.006. Epub 2016 Jul 21.
The present in situ study investigated the effect of Inula viscosa tea on the pellicle's acid protective properties and on initial oral biofilm formation.
Biofilm formation was performed on bovine enamel slabs on individual maxillary splints. Following 1min of pellicle formation, eight subjects rinsed for 10min with Inula viscosa tea and the splints remained for 8h intraorally. Samples carried after 1-min rinsing with CHX (0.2%) or without rinse served as controls. BacLight™ staining, 4',6-diamidino-2-phenylindole (DAPI)-staining and fluorescence in situ hybridization (FISH) were used for fluorescence microscopic detection of adherent bacteria. For investigation of acid protective properties, three subjects rinsed for 10min with Inula viscosa tea after 1min pellicle formation and kept the splints intraorally for further 19min. Physiological 30-min pellicles and native enamel samples served as controls. After HCl incubation of the samples ex-vivo over 120s (pH 2.0, 2.3, 3.0) calcium- and phosphate release were quantified photometrically. Potential influences on the pellicle's ultrastructure by Inula viscosa tea were evaluated by transmission electron microscopy (TEM).
Application of Inula viscosa tea yielded a significant reduction of adherent bacteria on all enamel samples as detected by fluorescence microscopy. For calcium- and phosphate release no significant effect was recorded. TEM investigation indicated a modification of the pellicle's ultrastructure, but no enhanced protection against erosive noxae.
Rinsing with Inula viscosa tea influences the bacterial colonization on enamel in situ over 8h but has no impact on acid protective properties of the pellicle.
本原位研究调查了旋覆花茶对获得性膜的酸保护特性及初始口腔生物膜形成的影响。
在个体上颌夹板的牛牙釉质板上进行生物膜形成。在获得性膜形成1分钟后，8名受试者用旋覆花茶漱口10分钟，夹板在口腔内放置8小时。用0.2%氯己定（CHX）冲洗1分钟或不冲洗的样本作为对照。使用BacLight™ 染色、4',6-二脒基-2-苯基吲哚（DAPI）染色和荧光原位杂交（FISH）对黏附细菌进行荧光显微镜检测。为了研究酸保护特性，3名受试者在获得性膜形成1分钟后用旋覆花茶漱口10分钟，并将夹板在口腔内再放置19分钟。生理状态下30分钟的获得性膜和天然牙釉质样本作为对照。在体外将样本用盐酸孵育120秒（pH 2.0、2.3、3.0）后，通过光度法对钙和磷的释放进行定量。通过透射电子显微镜（TEM）评估旋覆花茶对获得性膜超微结构的潜在影响。
通过荧光显微镜检测发现，使用旋覆花茶后所有牙釉质样本上的黏附细菌显著减少。对于钙和磷的释放，未记录到显著影响。TEM研究表明获得性膜的超微结构发生了改变，但对侵蚀性刺激物没有增强的保护作用。
用旋覆花茶漱口在8小时内会影响牙釉质上的细菌定植，但对获得性膜的酸保护特性没有影响。
