Khatter J C, Agbanyo M, Navaratnam S
Department of Medicine, University of Manitoba, Winnipeg, Canada.
Life Sci. 1991;48(5):387-96. doi: 10.1016/0024-3205(91)90493-u.
In the past few years, we developed an extraction procedure which we successfully used to isolate a crude fraction containing digitalis-like substance (DLS) from porcine left ventricular tissue. In this study, the crude fraction was found to cross-react with digoxin antibodies and showed immunoreactivity of 4.25 +/- 0.6 ng digoxin equivalent/ml. On further purification of the crude fraction using silica gel G column chromatography, a fraction C was obtained, which was highly positive inotropic on canine trabeculae and it dose-dependently inhibited ouabain sensitive 86Rb+ uptake in rat heart slices. A 50% inhibition of uptake was obtained by 25 microliters of fraction C. Fraction C also inhibited canine kidney Na+, K(+)-ATPase (Sigma, U.S.A.) dose-dependently and a 50% inhibition of this enzyme required 17 microliters of fraction C. Ashing of the fraction C at 500 degrees C resulted in loss of inotropic and enzyme inhibitory activities, indicating an organic nature of the unknown digitalis-like substance.
在过去几年里，我们开发了一种提取方法，并成功地用该方法从猪左心室组织中分离出一种含有洋地黄样物质（DLS）的粗提物。在本研究中，发现该粗提物与地高辛抗体发生交叉反应，免疫反应性为4.25±0.6纳克地高辛当量/毫升。使用硅胶G柱色谱对粗提物进一步纯化后，得到了组分C，其对犬小梁具有高度正性肌力作用，并且对大鼠心脏切片中哇巴因敏感的86Rb+摄取具有剂量依赖性抑制作用。25微升的组分C可使摄取抑制50%。组分C还对犬肾Na+，K(+)-ATP酶（美国西格玛公司）具有剂量依赖性抑制作用，抑制该酶50%需要17微升的组分C。组分C在500℃灰化导致正性肌力和酶抑制活性丧失，表明未知洋地黄样物质具有有机性质。
