Khatter J C, Agbanyo M, Hoeschen R J
Life Sci. 1986 Dec 22;39(25):2483-92. doi: 10.1016/0024-3205(86)90491-1.
A crude fraction was isolated from pig heart left ventricle (150 g) homogenates after extraction of lipids, chromatographic separation and desalting. The extract contained an ionic content of 0.21, 0.27, 0.33 and 1.7 mM respectively for Mg2+, Ca2+, K+, and Na+. The albumin extract, used as a reference control, contained an ionic content of 0.88 and 2.1 mM respectively for K+ and Na+ and negligible amounts of Mg2+ and Ca2+. The isolated fraction exhibited digitalis-like properties in the inhibition of sarcolemmal Na+, K+-ATPase in a dose dependent manner, the displacement of [3H]-ouabain binding from membrane receptor sites and produced +ve inotropic response in isolated perfused heart in a dose dependent manner. The albumin extract tested in the same manner showed no digitalis-like properties. The ventricular fraction was unable to displace (-) 3H-DHA binding from membrane sites and its inotropic action was not blocked by propranolol. The data suggests that the fraction isolated from pig heart left ventricle contains a substance which has some properties like digitalis.
从猪心脏左心室（150克）匀浆中提取脂质、进行色谱分离和脱盐后，分离出一种粗提物。提取物中镁离子、钙离子、钾离子和钠离子的离子含量分别为0.21、0.27、0.33和1.7毫摩尔。用作参考对照的白蛋白提取物中，钾离子和钠离子的离子含量分别为0.88和2.1毫摩尔，镁离子和钙离子的含量可忽略不计。分离出的组分呈现出洋地黄样特性，能以剂量依赖性方式抑制肌膜钠钾ATP酶，从膜受体位点置换[3H]哇巴因结合，并在离体灌注心脏中以剂量依赖性方式产生正性肌力反应。以相同方式测试的白蛋白提取物未显示出洋地黄样特性。心室组分无法从膜位点置换（-）3H-DHA结合，其正性肌力作用也未被普萘洛尔阻断。数据表明，从猪心脏左心室分离出的组分含有一种具有类似洋地黄某些特性的物质。
