Genome. 1993 Feb;36(1):131-8. doi: 10.1139/g93-016.
Electron microscopy of whole-mount surface-spread synaptonemal complex complements and conventional light microscopy of chromosomes at first metaphase of meiosis were used to compare the relative frequencies of pairing configurations at the two stages in inbred autotetraploid rye (Secale cereale L.). Statistical tests showed significantly fewer multivalents at first metaphase than expectations based on random initiation of synapsis at each telomeric site within each group of four homologues. Direct observations of synaptic behaviour of chromosomes showed that this deviation is due primarily to a preponderance of bivalents during zygotene and pachytene. It is also the result of a significant drop in multivalent frequency from meiotic prophase to metaphase I, which is attributable both to a lack of chiasmata with which to consolidate multivalents and inhibition of chiasma formation in synaptonemal complex segments of multivalents that are nonhomologous.
电镜下对铺展的整体联会复合体的观察和减数分裂第一次分裂中期染色体的常规光镜检查，被用来比较自交四倍体黑麦（Secale cereale L.）在这两个阶段的配对构型的相对频率。统计检验表明，第一次分裂中期的多价体明显少于根据每个四联体同源组内每个端粒位点随机起始联会的预期值。染色体的直接观察表明，这种偏差主要是由于合线期和粗线期二价体的优势。这也是多价体频率从减数分裂前期到中期 I 显著下降的结果，这归因于缺乏可以巩固多价体的交叉和抑制非同源多价体联会复合体片段中的交叉形成。
