Genome. 1994 Oct;37(5):784-93. doi: 10.1139/g94-112.
The influence of chromosome structure upon pairing behaviour during meiosis was investigated by comparing four autotetraploid genotypes of rye (Secale cereale) containing homologous chromosome sets with different degrees of structural similarity. The series provided a range of genotypes that, at one extreme, contained structurally identical chromosome sets and, at the other extreme, sets that are certainly more heterozygous in the genic sense and probably also more diverse from a purely structural viewpoint. Relative frequencies of pairing configurations at meiotic prophase and metaphase I were compared by electron microscopy of whole-mount surface-spread synaptonemal complex complements and light microscopy of squash preparations. Despite unexpectedly low quadrivalent frequencies over all four genotypes, higher mean bivalent frequencies appeared to be associated with greater homologue diversity. In other words, greater structural divergence between chromosome sets appears to facilitate more efficient discrimination between homologous and identical chromosomes that drives the formation of bivalents. Statistical comparisons were not able to confirm in some cases the significance of the observed pattern of pairing behaviour.
通过比较四个含有同源染色体组的不同结构相似程度的四倍体黑麦（Secale cereale）的自交系，研究了染色体结构对减数分裂中配对行为的影响。该系列提供了一系列的基因型，一端包含结构完全相同的染色体组，另一端则在基因意义上肯定更为杂合，而且从纯粹的结构角度来看也可能更为多样化。通过对整个表面展开联会复合体补体的电子显微镜和压片的光镜检查，比较了减数分裂前期和中期 I 的配对构型的相对频率。尽管在所有四个基因型中四价体的频率都出乎意料地低，但较高的平均二价体频率似乎与更大的同源体多样性有关。换句话说，染色体组之间更大的结构差异似乎有助于更有效地区分同源和相同的染色体，从而形成二价体。在某些情况下，统计比较无法证实所观察到的配对行为模式的显著性。
