Genome. 1994 Apr;37(2):264-70. doi: 10.1139/g94-037.
The genome size (1C value) and base composition of 14 ecotypes of two species of tetraploid and diploid Medicago have been assessed by flow cytometry. These parameters vary both between and within species. The diploid annual Medicago truncatula Gaertn. had the smallest genome of the group studied (which also covered M. sativa L. subsp. sativa, M. sativa L. subsp. caerulea (Less. ex Ledeb.) Schmalh., M. sativa L. subsp. quasifalcata Sinsk., M. sativa L. subsp. x varia (Martyn) Arcangeli; however, its ecotypes revealed substantial intraspecific variation. The smallest M. truncatula genome observed was ecotype 108-1 with 1C = 0.49 pg and 38.1% GC and the largest was Jemalong with 1C = 0.57 pg and 38.6% GC. The degree of polysomaty in these Medicago was low, although in some tissues the frequency of cells with 4C nuclei reached 50%.
通过流式细胞术评估了 14 个四倍体和二倍体 Medicago 物种的 14 个生态型的基因组大小（1C 值）和碱基组成。这些参数在种间和种内均有差异。研究组涵盖了 M. sativa L. subsp. sativa、M. sativa L. subsp. caerulea (Less. ex Ledeb.) Schmalh.、M. sativa L. subsp. quasifalcata Sinsk.、M. sativa L. subsp. x varia (Martyn) Arcangeli，其中二倍体一年生 Medicago truncatula Gaertn. 的基因组最小，但它的生态型显示出很大的种内变异。观察到的最小的 M. truncatula 基因组是 108-1 生态型，其 1C 值为 0.49 pg，GC 含量为 38.1%，最大的是 Jemalong，其 1C 值为 0.57 pg，GC 含量为 38.6%。这些 Medicago 的多倍体程度较低，尽管在某些组织中，具有 4C 核的细胞频率达到 50%。
