Genome. 1994 Aug;37(4):577-83. doi: 10.1139/g94-082.
Polymerase chain reaction (PCR) based random amplified polymorphic DNA (RAPD) markers were used to study the extent of redundancy (duplication of genetic materials) within a genetic resources collection. Nine nearly phenotypically and identical accessions of butterhead lettuce (Lactuca sativa L.) were assayed for their genetic identities. A nonuniform, heterogeneous butterhead line and a crisphead cultivar were added for population comparison. PCR amplification using 13 oligonucleotide primers generated 93 polymorphic bands. The percentage of segregating bands was used to determine within-line variation; values ranged from 0.0 to 12.0%, except for the nonuniform line at 22.6%. Between-line similarity was measured using similarity coefficients and ranged from 0.919 to 0.985. The relationship between the crisphead accession and a composite of all butterhead accessions was 0.84. Selfed progeny of each line were measured for morphological uniformity. The variation obtained from these biological data was compared with variation detected at the DNA level and each was positively correlated. Results demonstrate that RAPD analyses may serve as a major source of information for separation of closely related accessions, especially when integrated with phenotypic measures.
聚合酶链式反应（PCR）基础的随机扩增多态性 DNA（RAPD）标记被用于研究遗传资源收集内冗余（遗传物质的重复）的程度。对 9 个几乎表型和相同的奶油生菜（生菜）品种进行了遗传同一性分析。为了种群比较，还添加了一条非均匀的、异质的奶油生菜和一个脆生菜品种。使用 13 个寡核苷酸引物进行 PCR 扩增，产生了 93 个多态性条带。分离带的百分比用于确定线内变异；除了 22.6%的非均匀线外，值范围为 0.0 到 12.0%。使用相似系数测量线间相似性，范围从 0.919 到 0.985。脆生菜品种与所有奶油生菜品种的综合品种的关系为 0.84。每条线的自交后代都进行了形态均匀性测量。从这些生物学数据中获得的变异与在 DNA 水平上检测到的变异进行了比较，两者呈正相关。结果表明，RAPD 分析可以作为分离密切相关品种的主要信息来源，尤其是与表型测量相结合时。
