Genome. 1994 Aug;37(4):607-12. doi: 10.1139/g94-086.
Genomic fluorescent in situ hybridization was employed in the study of the genome organization and evolution of hexaploid oat (Avena sativa L. cv. Sun II, AACCDD, 2n = 6x = 42). Genomic DNAs from two diploid oat species, Avena strigosa (genomic constitution AsAs, 2n = 14) and Avena pilosa (genomic constitution CpCp, 2n = 14), were used as probes in the study. The DNA from A. strigosa labelled 28 of the 42 (2/3) chromosomes of the hexaploid oat, while 14 of the 42 (1/3) chromosomes were labelled with A. pilosa DNA, indicating a close relationship between the A and D genomes. Results also suggested that at least 18 chromosomes (9 pairs) were involved in intergenomic interchanges between the A and C genomes.
本研究利用基因组荧光原位杂交技术研究了六倍体燕麦（Avena sativa L. cv. Sun II，AACCDD，2n = 6x = 42）的基因组结构和进化。研究中使用了两种二倍体燕麦物种的基因组 DNA，即 A. strigosa（基因组组成 AsAs，2n = 14）和 A. pilosa（基因组组成 CpCp，2n = 14）作为探针。A. strigosa 的 DNA 标记了六倍体燕麦的 42 条染色体中的 28 条（2/3），而 42 条染色体中的 14 条（1/3）被 A. pilosa DNA 标记，这表明 A 和 D 基因组之间存在密切关系。结果还表明，A 和 C 基因组之间至少有 18 条染色体（9 对）发生了基因组间的相互交换。
