Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Julius Kuehn Institute (JKI), Rudolf-Schick-Platz 3a, OT Gross Lüsewitz, 18190, Sanitz, Germany.
I.G. Saatzucht GmbH & Co KG, Am Park 3, 18276, Gülzow-Prüzen OT Boldebuck, Germany.
Theor Appl Genet. 2023 Mar 13;136(3):53. doi: 10.1007/s00122-023-04288-z.
Three independent experiments with different genetic backgrounds mapped the resistance gene Pm7 in the oat genome to the distal part of the long arm of chromosome 5D. Resistance of oat to Blumeria graminis DC. f. sp. avenae is an important breeding goal in Central and Western Europe. In this study, the position of the effective and widely used resistance gene Pm7 in the oat genome was determined based on three independent experiments with different genetic backgrounds: genome-wide association mapping in a diverse set of inbred oat lines and binary phenotype mapping in two bi-parental populations. Powdery mildew resistance was assessed in the field as well as by detached leaf tests in the laboratory. Genotyping-by-sequencing was conducted to establish comprehensive genetic fingerprints for subsequent genetic mapping experiments. All three mapping approaches located the gene to the distal part of the long arm of chromosome 5D in the hexaploid oat genome sequences of OT3098 and 'Sang.' Markers from this region were homologous to a region of chromosome 2Ce of the C-genome species, Avena eriantha, the donor of Pm7, which appears to be the ancestral source of a translocated region on the hexaploid chromosome 5D.
三个具有不同遗传背景的独立实验将燕麦中的抗病基因 Pm7 定位到 5D 染色体长臂的远端。燕麦对禾本科布氏黑粉菌的抗性是中欧和西欧的一个重要育种目标。在这项研究中，基于三个具有不同遗传背景的独立实验，确定了燕麦基因组中有效且广泛使用的抗病基因 Pm7 的位置：在一组多样化的近交系燕麦品系中进行全基因组关联作图，以及在两个双亲群体中进行二元表型作图。在田间和实验室的离体叶片试验中评估了白粉病的抗性。通过测序进行基因分型，为随后的遗传作图实验建立了全面的遗传指纹图谱。所有三种作图方法都将该基因定位到六倍体燕麦基因组 OT3098 和 'Sang' 的 5D 染色体长臂的远端。该区域的标记与 Pm7 的供体二倍体物种 A. eriantha 的 2Ce 染色体的一个区域同源，该区域似乎是六倍体染色体 5D 上易位区域的祖先来源。
