野豌豆染色体 cDNA 序列的分布。
The distribution of cDNA sequences on field bean chromosomes.
出版信息
Genome. 1994 Dec;37(6):1065-7. doi: 10.1139/g94-152.
DOI:10.1139/g94-152
PMID:18470146
Abstract
Total polyadenylated RNA was isolated from different organs of the field bean by oligo-dT latex particles. Complementary DNA (cDNA) was reverse transcribed from the mRNAs and used as a probe for fluorescent in situ hybridization to metaphase chromosomes. Hybridization signals covered the whole chromosome complement except for late-replicating Giemsa-banded heterochromatin regions, which are composed mainly (or exclusively) of tandemly arranged repetitive sequences.
摘要
总多聚腺苷酸 RNA 是从小扁豆的不同器官中通过 oligo-dT 乳胶颗粒分离得到的。将来自 mRNAs 的互补 DNA(cDNA)反转录并用作荧光原位杂交到中期染色体的探针。杂交信号覆盖了除了 late-replicating Giemsa 带型异染色质区域以外的整个染色体组，这些区域主要（或完全）由串联排列的重复序列组成。
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