将信使核糖核酸（mRNA）的特定序列定位到人类染色体的浅染G带。
Localization of sequences specifying messenger RNA to light-staining G-bands of human chromosomes.
作者信息
Yunis J J, Kuo M T, Saunders G F
出版信息
Chromosoma. 1977 Jun 23;61(4):335-44. doi: 10.1007/BF00288617.
PMID:880841
Abstract
Total cytoplasmic polyadenylated RNA was isolated from the human lymphocyte cell line Wil2 by oligo(dT)-cellulose chromatography. Tritiated complementary DNA (cDNA) was transcribed from the RNA and used as a probe for in situ hybridization to metaphase chromosomes. The majority of G-negative or lightly staining bands were found to be preferential sites of hybridization.
摘要
通过寡聚（dT）-纤维素色谱法从人淋巴细胞系Wil2中分离出总细胞质多聚腺苷酸化RNA。用该RNA转录出氚标记的互补DNA（cDNA），并将其用作中期染色体原位杂交的探针。发现大多数G阴性或浅染带是优先杂交位点。
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