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利用牛内皮细胞和鸡胚检测蓝舌病病毒。

Detection of bluetongue virus by using bovine endothelial cells and embryonated chicken eggs.

作者信息

Wechsler S J, Luedke A J

机构信息

Arthropod-borne Animal Diseases Research Laboratory, U.S. Department of Agriculture, Laramie, Wyoming 82071-3965.

出版信息

J Clin Microbiol. 1991 Jan;29(1):212-4. doi: 10.1128/jcm.29.1.212-214.1991.

Abstract

Two systems, inoculation of bovine endothelial cells and of embryonated chicken eggs, were compared for detection of bluetongue virus (BTV) in blood specimens from experimentally inoculated sheep. For all BTV serotypes tested, embryonated chicken eggs detected longer periods of viremia than did bovine endothelial cells, primarily by detecting BTV in samples containing lower virus concentrations.

摘要

为了检测实验接种绵羊血液样本中的蓝舌病毒(BTV),对两种系统进行了比较,即牛内皮细胞接种系统和鸡胚接种系统。对于所有测试的BTV血清型,鸡胚检测到病毒血症的时间比牛内皮细胞更长,主要是通过检测病毒浓度较低的样本中的BTV来实现的。

相似文献

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Bluetongue: laboratory diagnosis.蓝舌病:实验室诊断
Comp Immunol Microbiol Infect Dis. 1994 Aug-Nov;17(3-4):221-42. doi: 10.1016/0147-9571(94)90045-0.

本文引用的文献

6
Bluetongue in sheep: viral assay and viremia.
Am J Vet Res. 1969 Apr;30(4):499-509.
7
An overview of diagnostics for bluetongue.
Prog Clin Biol Res. 1985;178:423-33.
8
Susceptibilities of 14 cell lines to bluetongue virus infection.14种细胞系对蓝舌病毒感染的易感性。
J Clin Microbiol. 1988 Nov;26(11):2324-7. doi: 10.1128/jcm.26.11.2324-2327.1988.
9
Limits of detection of bluetongue virus with different assay systems.
J Vet Diagn Invest. 1990 Apr;2(2):103-6. doi: 10.1177/104063879000200203.

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