Rainwater R, Mann K
Biology Department, University of Alaska, Anchorage 99508.
Virology. 1991 Mar;181(1):408-11. doi: 10.1016/0042-6822(91)90515-d.
The subcellular localization of topoisomerase I and topoisomerase II has been compared in Simian virus (SV40)-infected and uninfected TC7 monkey cells. In SV40-infected cells, both of these enzymes are preferentially associated with the chromatin. Some topoisomerase I is associated with the nuclear matrix, whereas topoisomerase II shows no such association. In uninfected TC7 cells, topoisomerase I is present in both the chromatin and nuclear matrix fractions. Topoisomerase II, on the other hand, is not detected in any of the subcellular fractions of uninfected cells. After SV40 infection, there is a marked increase in the level of chromatin-associated topoisomerase II.
在感染猿猴病毒（SV40）和未感染的TC7猴细胞中，对拓扑异构酶I和拓扑异构酶II的亚细胞定位进行了比较。在感染SV40的细胞中，这两种酶都优先与染色质相关联。一些拓扑异构酶I与核基质相关，而拓扑异构酶II则没有这种关联。在未感染的TC7细胞中，拓扑异构酶I存在于染色质和核基质组分中。另一方面，在未感染细胞的任何亚细胞组分中均未检测到拓扑异构酶II。SV40感染后，与染色质相关的拓扑异构酶II水平显著增加。
