Rainwater R, Mann K
Biology Department, University of Alaska, Anchorage 99508.
J Virol. 1990 Feb;64(2):918-21. doi: 10.1128/JVI.64.2.918-921.1990.
The time course of expression of topoisomerase I, topoisomerase II, and simian virus 40 (SV40) large tumor (T) antigen was determined in whole-cell extracts of uninfected versus SV40-infected TC7 cells. After a minor increase, the level of topoisomerase I remained fairly constant throughout the time course in both uninfected and SV40-infected cells. In contrast, the level of topoisomerase II increased markedly in SV40-infected cells but not in uninfected cells following the appearance of SV40 T antigen.
在未感染与感染猿猴病毒40（SV40）的TC7细胞的全细胞提取物中，测定了拓扑异构酶I、拓扑异构酶II和猿猴病毒40（SV40）大T抗原的表达时间进程。经过轻微增加后，拓扑异构酶I的水平在未感染和感染SV40的细胞的整个时间进程中都保持相当恒定。相比之下，在SV40 T抗原出现后，拓扑异构酶II的水平在感染SV40的细胞中显著增加，但在未感染的细胞中没有增加。
