菜蓟素 A 对体外培养的小鼠腹腔巨噬细胞和兔滑膜细胞前列腺素 E(2)生成的抑制作用。
Inhibitory effect of esculentoside A on prostaglandin E(2) production from murine peritoneal macrophages and rabbit synovial cells in vitro.
机构信息
Department of Pharmacology College of Pharmacy Second Military Medical University Shanghai 200433 China.
出版信息
Mediators Inflamm. 1997;6(1):22-4. doi: 10.1080/09629359791884.
Esculentoside A (EsA) is a saponin isolated from the roots of Phytolacca esculenta. Previous experiments have shown that it has strong antiinflammatory effects. To investigate the mechanism of anti-inflammatory effects of esculentoside A (EsA),[(3)H] arachidonic acid (AA) prelabelled murine macrophage and radioimmunoassay were used to test the effect of EsA on the total release of AA and prostaglandin E(2) in culture supernatants. The results showed that EsA had no significant effect on the total release of AA from murine macrophages. EsA (2.5-10 mumol/l), from unstimulated murine peritoneal macrophages and rabbit synovial cells, could decrease the production of prostaglandin E(2). In A(23187) and LPS-treated macrophages and synovial cells, EsA (10 mumol/l) could significantly decrease the prostaglandin E(2) production. These results confirmed that EsA exerted an inhibitory effect on prostaglandin E(2) production from murine macrophages and rabbit synovial cells.
Esculentoside A（EsA）是从商陆根部分离得到的一种皂苷。先前的实验表明，它具有很强的抗炎作用。为了研究 Esculentoside A（EsA）的抗炎作用机制，我们使用[（3）H]花生四烯酸（AA）标记的鼠巨噬细胞和放射免疫测定法来测试 EsA 对培养上清液中 AA 总释放和前列腺素 E（2）的影响。结果表明，EsA 对鼠巨噬细胞 AA 的总释放没有明显影响。EsA（2.5-10μmol/L）可从未刺激的鼠腹腔巨噬细胞和兔滑膜细胞中减少前列腺素 E（2）的产生。在 A（23187）和 LPS 处理的巨噬细胞和滑膜细胞中，EsA（10μmol/L）可显著减少前列腺素 E（2）的产生。这些结果证实，EsA 对鼠巨噬细胞和兔滑膜细胞中前列腺素 E（2）的产生具有抑制作用。
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