Sharma Rakesh, Katz Jose K
Department of Radiology and Medicine, Columbia University, New York, NY 10032, USA.
Technol Cancer Res Treat. 2008 Jun;7(3):175-85. doi: 10.1177/153303460800700303.
Increased PET and MRI image intensities of mouse prostate tumors were correlated with histostaining tumor characteristics. The hypothesis was that increased intracellular sodium microMRI signal intensities and flouro-2-deoxy-glucose utilization by microPET in apoptosis rich regions in tumors were positively correlated as chemosensitivity assay of Taxotere. The PC-3 cancer cell line induced prostate tumor MRI and PET images and histology slices were digitally captured and compared in pre- and post-Taxotere treated tumors. The optimization of inversion recovery MRI parameters was done to generate sodium images of phantom. The (18)FDG biotransformation was optimized to measure PET image intensities. A criterion was developed to evaluate malignancy by histology. For correlation, regression analysis was done using imaging, histology, and immunostaining data from PC3 tumor after 24 and 48 hours post-Taxotere treatment. Apoptosis indices were calculated by histostaining and ss-DNA antibody assay. Sodium MRI and PET signal intensity distributions were comparable at specific locations relatively and measured in tumor tissue regions. In tumors, Taxotere induced an increase in intracellular sodium MRI signal 30% (p<0.001) with decreased tumor size (20%; p<0.001) and micro-PET showed FDG uptake increase 15% (p<0.001) with decreased tumor size (10%; p<0.001) than that of control tumors after 24 hours. Histological features indicated tumor risk (high 'intracellular/extracellular ratio', high mitotic index, and apoptotic index), decreased tumor viability (reduced mitotic figures, reduced diploidy or aneuploidy, and proliferation index) after Taxotere treatment. These features in co-registered intracellular sodium, microPET hypermetabolic, and monoclonal antibody (ss-DNA) sensitive regions showed (% difference > 6%). Apoptosis rich regions showed characteristic nuclei with S phase DNA histogram, appearing brighter on IC-Na images and mild active on PET images (sensitivity=65%; specificity=70%). In conclusion, MRI and PET multimodal imaging may be rapid non-invasive chemosensitivity assay to monitor the drug anticancer effect.
小鼠前列腺肿瘤的PET和MRI图像强度增加与组织染色的肿瘤特征相关。假设是肿瘤中富含凋亡区域的细胞内钠微MRI信号强度增加与微PET的氟代-2-脱氧葡萄糖利用率增加呈正相关，如同多西他赛的化学敏感性测定。PC-3癌细胞系诱导的前列腺肿瘤的MRI和PET图像以及组织学切片在多西他赛治疗前后进行了数字采集和比较。对反转恢复MRI参数进行了优化以生成体模的钠图像。对(18)FDG生物转化进行了优化以测量PET图像强度。制定了一个通过组织学评估恶性程度的标准。为了进行相关性分析，使用多西他赛治疗后24小时和48小时PC3肿瘤的成像、组织学和免疫染色数据进行了回归分析。通过组织染色和单链DNA抗体测定计算凋亡指数。钠MRI和PET信号强度分布在肿瘤组织区域的特定位置相对可比并进行了测量。在肿瘤中，多西他赛治疗24小时后，与对照肿瘤相比，细胞内钠MRI信号增加30%（p<0.001），肿瘤大小减小20%（p<0.001），微PET显示FDG摄取增加15%（p<0.001），肿瘤大小减小10%（p<0.001）。组织学特征表明肿瘤风险（高“细胞内/细胞外比率”、高有丝分裂指数和凋亡指数），多西他赛治疗后肿瘤活力降低（有丝分裂图像减少、二倍体或非整倍体减少以及增殖指数降低）。在共配准的细胞内钠、微PET高代谢和单克隆抗体（单链DNA）敏感区域的这些特征显示（差异百分比>6%）。富含凋亡的区域显示出具有S期DNA直方图的特征性细胞核，在细胞内钠图像上显得更亮，在PET图像上轻度活跃（敏感性=65%；特异性=70%）。总之，MRI和PET多模态成像可能是一种快速的非侵入性化学敏感性测定方法，用于监测药物的抗癌效果。
