Tanaka Yasuhito, Sanchez Laura V, Sugiyama Masaya, Sakamoto Tomoyuki, Kurbanov Fuat, Tatematsu Kanako, Roman Sonia, Takahashi Satoru, Shirai Tomoyuki, Panduro Arturo, Mizokami Masashi
Department of Clinical Molecular Informative Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya, 467-8601 Japan.
Virology. 2008 Jul 5;376(2):408-15. doi: 10.1016/j.virol.2008.04.001. Epub 2008 May 13.
Accumulated evidence indicated that hepatitis B virus genotype G (HBV/G) is present exclusively in coinfection with other HBV genotypes. In Mexico, HBV/G from 6 men who had sex with men were coinfected with HBV/H. Phylogenetically complete genomes of the 6 Mexican HBV/G strains were closely related to previous ones from the US/Europe. Using uPA/SCID mice with human hepatocytes, monoinfection with HBV/G did not result in detectable HBV DNA in serum, whereas superinfection with HBV/G at week 10 inoculated HBV/H when HBV/H DNA was elevated to >10(7) copies/mL has enhanced the replication of HBV/G. The HBV/G was enhanced in another 3 inoculated with a serum passage containing HBV/G with a trace of HBV/H. Coinfection of mice with HBV/G and H induced fibrosis in the liver. In conclusion, the replication of HBV/G can be enhanced remarkably when it is coinfected with HBV/H. Coinfection with HBV/G may be directly cytopathic in immunosuppressive conditions.
越来越多的证据表明，乙型肝炎病毒G基因型（HBV/G）仅在与其他HBV基因型合并感染时出现。在墨西哥，6名男男性行为者的HBV/G与HBV/H合并感染。这6株墨西哥HBV/G毒株的系统发育完整基因组与之前来自美国/欧洲的毒株密切相关。使用植入人肝细胞的uPA/SCID小鼠，单独感染HBV/G不会导致血清中检测到HBV DNA，而在第10周接种HBV/H且HBV/H DNA升高至>10⁷拷贝/mL时再感染HBV/G，则会增强HBV/G的复制。另外3只接种含有微量HBV/H的HBV/G血清传代毒株的小鼠，其HBV/G复制也增强。小鼠同时感染HBV/G和HBV/H会导致肝脏纤维化。总之，HBV/G与HBV/H合并感染时，其复制可显著增强。在免疫抑制条件下，HBV/G合并感染可能具有直接细胞病变作用。
