从人类携带者中分离出的丙型肝炎病毒基因组的结构与组织
Structure and organization of the hepatitis C virus genome isolated from human carriers.
作者信息
Takamizawa A, Mori C, Fuke I, Manabe S, Murakami S, Fujita J, Onishi E, Andoh T, Yoshida I, Okayama H
机构信息
Research Foundation for Microbial Diseases of Osaka University, Kagawa, Japan.
出版信息
J Virol. 1991 Mar;65(3):1105-13. doi: 10.1128/JVI.65.3.1105-1113.1991.
Abstract
Hepatitis C virus (HCV) is a major causative agent of posttransfusion non-A, non-B hepatitis, which often develops into malignant chronic diseases, including liver cirrhosis and hepatocellular carcinoma. We have cloned from human carriers overlapping cDNAs (9,416 bp) covering the entire coding region of the HCV genome. The latter encodes a 3,010-amino-acid polyprotein. In addition, there are 332 and 54 bases of 5' and 3' noncoding sequences, respectively. Our HCV strain has a 77% nucleic acid identity to the HCV strain cloned by workers at Chiron Corporation. The hydrophobicity profile of the putative polyprotein is similar to those of flaviviruses, but it has limited amino acid homology to polyproteins of flaviviruses and other viruses, indicating that HCV is at most distantly related to flaviviruses.
摘要
丙型肝炎病毒（HCV）是输血后非甲非乙型肝炎的主要致病因子，这种肝炎常常会发展成恶性慢性疾病，包括肝硬化和肝细胞癌。我们已从人类携带者中克隆出覆盖HCV基因组整个编码区的重叠cDNA（9416 bp）。后者编码一种含3010个氨基酸的多聚蛋白。此外，5'和3'非编码序列分别有332个和54个碱基。我们的HCV毒株与Chiron公司工作人员克隆的HCV毒株有77%的核酸同一性。推测的多聚蛋白的疏水性图谱与黄病毒属的相似，但与黄病毒属和其他病毒的多聚蛋白的氨基酸同源性有限，这表明HCV与黄病毒属最多只有远缘关系。
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